Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-27712 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDw293 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, Neuro2A, C8D30, NIH-3T3, human BMPR1B-transfected 293T, Rat2.
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDw293/BMPR1B using Non-transfected (-) and BMPR1B-transfected (+,) 293T whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a CDw293/BMPR1B polyclonal antibody (Product # PA5-27712) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMPR1B in Non-transfected (-) and BMPR1B-transfected (+) 293T whole cell extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with BMPR1B Polyclonal antibody (Product # PA5-27712) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BMPR1B in Various whole cell extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with BMPR1B Polyclonal antibody (Product # PA5-27712) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDw293 was performed by separating 30 µg of non-transfected (-) and BMPR1B-transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDw293 Polyclonal Antibody (Product # PA5-27712) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BMPR1B antibody detects BMPR1B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BMPR1B antibody CDw293 Polyclonal Antibody (Product # PA5-27712) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDw293 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDw293 Polyclonal Antibody (Product # PA5-27712) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDw293 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDw293 Polyclonal Antibody (Product # PA5-27712) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of CDw293 was performed in frozen sectioned adult mouse retina tissue using CDw293 Polyclonal Antibody (Product # PA5-27712) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDw293 Polyclonal Antibody detects BMPR1B protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. CDw293 Polyclonal Antibody (Product # PA5-27712) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.