AM09036PU-S

antibody from OriGene

Targeting: STEAP1 PRSS24, STEAP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM09036PU-S

Provider

OriGene

Product name

STEAP1 mouse monoclonal antibody, clone J2D2, Purified

Antibody type

Monoclonal

Description

STEAP1 mouse monoclonal antibody, clone J2D2, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

STEAP1

Isotype

IgG

Antibody clone number

J2D2

Vial size

50 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis: Cell lysates of LNcap (30 ug) and Rat testis (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 antibody (Clone J2D2) (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence staining of human LNCap cell colony with monoclonal anti- human STEAP1 TEAP1 antibody (Clone J2D2)

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 antibody (Clone J2D2) (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Validation comment

IHC