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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB2051 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2051, RRID:AB_1714941
- Product name
- STEAP1 monoclonal antibody, clone J2D2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant STEAP1.
- Isotype
- IgG
- Antibody clone number
- J2D2
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo.
Recognition of six-transmembrane epithelial antigen of the prostate-expressing tumor cells by peptide antigen-induced cytotoxic T lymphocytes.
Challita-Eid PM, Morrison K, Etessami S, An Z, Morrison KJ, Perez-Villar JJ, Raitano AB, Jia XC, Gudas JM, Kanner SB, Jakobovits A
Cancer research 2007 Jun 15;67(12):5798-805
Cancer research 2007 Jun 15;67(12):5798-805
Recognition of six-transmembrane epithelial antigen of the prostate-expressing tumor cells by peptide antigen-induced cytotoxic T lymphocytes.
Rodeberg DA, Nuss RA, Elsawa SF, Celis E
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Jun 15;11(12):4545-52
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Jun 15;11(12):4545-52
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Cell lysates of LNCap (Lane 1, 30 ug) and rat testis (Lane 2, 30 ug) were resolved by SDS-PAGE and probed with STEAP1 monoclonal antibody, clone J2D2 (Cat # MAB2051) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human LNCaP cell colony with STEAP1 monoclonal antibody, clone J2D2 (Cat # MAB2051).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human breast cancer (A) and prostate hyperplasia tissue (B) were incubated with STEAP1 monoclonal antibody, clone J2D2 (Cat # MAB2051) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
