GTX628547-01

antibody from GeneTex

Targeting: DKK1 DKK-1, SK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX628547-01

Provider

GeneTex

Product name

DKK1 antibody [EPR4759]

Antibody type

Monoclonal

Host

Rabbit

Vial size

100 µl

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GST antibody immunoprecipitates GST-fused protein in IP experiments.IP Sample: GST-fused protein expressed E. coli lysate A. total lysate B. Control with 1 ?g of preimmune goat IgGC. Immunoprecipitation of GST-fused protein by 1 ?g of GST antibody (GTX26633)12% SDS-PAGE The immunoprecipitated GST-fuesd protein was detected by GST antibody (GTX26633) diluted at 1:10000. EasyBlot anti-goat IgG (GTX628547-01) was used as a secondary reagent.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

EasyBlot anti-Goat IgG (HRP)Comparison of western blot analysis with traditional secondary antibody (GTX228416-01 diluted at 1:5000) (1) and EasyBlot anti-Goat IgG kit (GTX628547-01 diluted at 1:2000) (2).A. 0.2 µg native goat IgGB. 0.1 µg native goat IgGC. 1 µg denatured goat IgGD. 2 µg denatured goat IgGE. 4 µg denatured goat IgGF. 8 µg denatured goat IgG

Validation comment

WB