21112-1-AP

antibody from Proteintech Group

Targeting: DKK1 DKK-1, SK

Provider product page for 21112-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [70]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 21112-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21112-1-AP, RRID:AB_10733097
Product name: DKK1 antibody
Antibody type: Polyclonal
Description: KD/KO validated DKK1 antibody (Cat. #21112-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DKK1 protein structure - 21112-1-AP shown in red.

Submitted references Decoding the DKK1/CKAP4 signaling Axis: A novel mechanism driving Neointimal hyperplasia in arteriovenous fistulas.

Xu S, Liang L, Xu H, Wang Q, Wei K, Wang Z, Kong X
International immunopharmacology 2026 May 15;177:116520

Exploring the role of metformin in high fluoride ingestion-induced bone lesions.

Xu W, Jiang N, Zhang Z, Xu H
Toxicology and applied pharmacology 2026 Jan;506:117653

Adipose-derived stem cells (ADSCs)-derived DKK1 promotes cutaneous wound healing by inducing M2 macrophage polarization via PI3K/AKT and JNK activation.

Liu D, Liu N, Liu X, Liu Y, Zhang H, Zhou Z, Zheng J, Tian Y
Biochimica et biophysica acta. Molecular cell research 2026 Jan;1873(1):120077

Gao Q, Tang W, Mi Z, He S, Yang H, Wang F, Huang J, Zhang Y, Wen J, Li L, Luo H, Liu X, Zhai X, Zhao X, Zhang L, Niu T, Zheng Y
Cancer cell international 2026 Jan 7;26(1):69

METTL16 Inhibits Lens Epithelial Cells Function in Diabetic Cataract via m6A-Modified DKK1-Mediated Wnt/β-Catenin Signaling.

Zhang B, Sun Y, Jiang X, Hu Z, Wang X, Zhao YE
Investigative ophthalmology & visual science 2026 Jan 5;67(1):1

Osteocytic Lipocalin-2 regulates bone formation locally through iron-dependent ferroptosis and Wnt suppression.

Khanal V, Carroll M, Moradi F, Carter J, Zhong Y, Shashank CG, Sato AY, Allen RM, Wankhade UD, Dole NS
Cell death discovery 2026 Feb 25;12(1)

Ophiopogon japonicus-derived plant exosomes: a promising strategy for blocking the progression of diabetic vasculopathy to myocardial infarction.

Pan Y, Tan M, Li X, Chen Q, Tao J, Lin X, Zeng Z, Song Y, Jiang F
BMC complementary medicine and therapies 2026 Feb 21;26(1)

Identification and validation of disulfidptosis-related gene signature revealed DKK1 as a therapeutic target in head and neck squamous cell carcinoma.

Li Z, Xu W, Li Z, Yu Z, Wang J
Scientific reports 2026 Apr 13;

Exosomal miR-3529-3p increases the sensitivity of trastuzumab via Wnt/β-catenin signaling pathway by targeting HOOK3.

Zhu J, Lu Y, Zhang Q
International immunopharmacology 2025 Sep 23;162:115160

P62 acts as an intermediator in cadmium-induced osteocyte apoptosis and osteoporosis in mice.

Yu F, Liu L, Ali W, Ma Y, Song R, Zhao H, Zou H, Bian J, Yuan Y, Gu J, Tong X, Liu Z
Biochemical pharmacology 2025 Oct;240:117104

Fecal microbiota transplantation promotes hair growth through gut microbiome and metabolic regulation.

Tian Y, Jin W, Jin X, Wang Y, Wu R, Yu R, Jiang J, Zhu M
Life sciences 2025 Oct 15;379:123887

Biosynthesis of the Natural Antioxidant Mycosporine-Glycine and Its Anti-Photodamage Properties.

Wang K, Cao J, Yao M, Zhang Q, Lin H, Qin L, Zhang Z, Wei J, Zhang H, He Y, Qu C, Liu M, Miao J
Journal of agricultural and food chemistry 2025 May 7;73(18):11094-11109

Exploration of the pharmacological substance basis and efficacy evaluation of fufang she-she-cao (Hedyotis diffusa formula) in repressing gastric cancer.

Chen DH, Mao PJ, Diao WJ, Lu JQ, Wu SL, Li Q
Journal of ethnopharmacology 2025 May 12;347:119711

Tumor cells induce neural DKK1 expression to promote MDSC infiltration and subsequent T cell suppression.

Liu R, Shi X, Qian S, Sun Z, Dai H, Wu Y, Cao S, Luo J, Zhang Z
Cellular signalling 2025 Mar;127:111576

Vdr mediates Wnt signaling pathway to regulate odontoblasts differentiation during dentin apposition.

Wu Y, Zhu W, Wang L, Zhang W, Zhang K, Sun M, Guan J, Liu S, Liu Y
European journal of pharmacology 2025 Mar 15;991:177333

TOP2A Promotes Proliferation, Migration, and Inflammatory Response in M5-Treated Keratinocytes by Binding CTBP1 to Activate Wnt/β-Catenin Signaling.

Yang Y, Liu S, Xiao X
Cell biochemistry and biophysics 2025 Jun;83(2):2101-2113

Calcaratarin D, a labdane diterpenoid, attenuates bleomycin-induced pulmonary fibrosis by blocking Wnt/β-catenin signaling pathway.

Liao W, Ang Y, Kee ACL, Lim V, Lim AYH, Chai CLL, Wong WSF
Pharmacological research 2025 Jun;216:107756

Apelin Alleviated Endothelial-To-Mesenchymal Transition via Increasing BKCa in Diabetic Nephropathy.

Fan C, Liu Y, Chang J, Huang M, Gao Z, Wang H, Zheng X, Li M, Xu J, Yin J, Zeng X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jun 30;39(12):e70747

Enhancing immunotherapy efficacy in colorectal cancer: targeting the FGR-AKT-SP1-DKK1 axis with DCC-2036 (Rebastinib).

Chen X, Zeng Q, Yin L, Yan B, Wu C, Feng J, Wu Y, He J, Ding W, Zhong J, Shen Y, Zu X
Cell death & disease 2025 Jan 9;16(1):8

Aerobic exercise and metformin attenuate the cognitive impairment in an experimental model of type 2 diabetes mellitus: focus on neuroinflammation and adult hippocampal neurogenesis.

Padhy DS, Aggarwal P, Velayutham R, Banerjee S
Metabolic brain disease 2025 Jan 7;40(1):92

Stroma-derived Dickkopf-1 contributes to the suppression of NK cell cytotoxicity in breast cancer.

Lee S, Ricci B, Tran J, Eul E, Ye J, Ren Q, Clever D, Wang J, Wong P, Haas MS, Stewart SA, Ma CX, Fehniger TA, Faccio R
Nature communications 2025 Jan 30;16(1):1183

Effects of the α-ketoglutaric acid on the performance, hair follicle development and antioxidant capacity of Rex rabbits.

Wang X, Li S, Chen J, Liu L, Li F
Animal nutrition (Zhongguo xu mu shou yi xue hui) 2025 Dec;23:353-368

Identification of HCAR1 as a ferroptosis-related biomarker of gastric cancer based on a novel ferroptosis-related prognostic model and in vitro experiments.

Zhang H, Zhan J, Zhou J, Liu L, He Y, Le Y, Liu W, Zhou L, Liu Y, Xiang X
Carcinogenesis 2025 Apr 3;46(2)

Lipocalin-2 Regulates Osteocyte Ferroptosis and Osteocyte-Osteoblast Crosstalk via Wnt Signaling to Control Bone Formation.

Khanal V, Carroll M, Carter J, Zhong Y, Chikkamagaluru S, Sato A, Allen R, Wankhade U, Dole N
Research square 2025 Apr 29;

MIR155HG suppresses the osteogenic differentiation of bone marrow mesenchymal stem cells through regulating miR-155-5p and DKK1 expression.

Li W, Yang C, Xu J, Ran D, Wang C
Journal of orthopaedic surgery and research 2025 Apr 19;20(1):392

A novel hypoxia- and lactate metabolism-related prognostic signature to characterize the immune landscape and predict immunotherapy response in osteosarcoma.

Wang Y, Wang X, Liu Y, Xu J, Zhu J, Zheng Y, Qi Q
Frontiers in immunology 2024;15:1467052

Engineering a targeted and safe bone anabolic gene therapy to treat osteoporosis in alveolar bone loss.

Lin C, Yang YS, Ma H, Chen Z, Chen D, John AA, Xie J, Gao G, Shim JH
Molecular therapy : the journal of the American Society of Gene Therapy 2024 Sep 4;32(9):3080-3100

Acquired curved hair is caused by fusion of multiple hair matrix cells.

Horibe I, Izumi S, Ke Y, Tanahashi N, Takagi Y, Ishihara R, Nakano T, Sumiyoshi T, Nagaoka Y
Journal of dermatological science 2024 Mar;113(3):130-137

Gut microbial alterations in arginine metabolism determine bone mechanical adaptation.

Wang D, Cai J, Pei Q, Yan Z, Zhu F, Zhao Z, Liu R, Guo X, Sun T, Liu J, Tian Y, Liu H, Shao X, Huang J, Hao X, Chang Q, Luo Z, Jing D
Cell metabolism 2024 Jun 4;36(6):1252-1268.e8

Enhancing motor functional recovery in spinal cord injury through pharmacological inhibition of Dickkopf-1 with BHQ880 antibody.

González-Fernández C, González P, Maqueda A, Pérez V, Rodríguez FJ
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Jul;176:116792

DKK1 loss promotes endometrial fibrosis via autophagy and exosome-mediated macrophage-to-myofibroblast transition.

Zhang Z, Hu J
Journal of translational medicine 2024 Jul 3;22(1):617

CircTOP1 targeted regulation of PTBP1 expression promotes the progression of coronary artery calcification.

Hu H, Shen S, Wu J, Ma L
Experimental cell research 2024 Jul 15;440(2):114147

Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics.

Shao X, Tian Y, Liu J, Yan Z, Ding Y, Hao X, Wang D, Shen L, Luo E, Guo XE, Luo P, Luo W, Cai J, Jing D
Nature communications 2024 Jan 30;15(1):890

Dickkopf-1 is an immune infiltration-related prognostic biomarker of head and neck squamous cell carcinoma.

Zhao C, Liu L, He Q, Li Y, Feng J, Chen Y, Li Y, Xu X, Zhu S, Ye Y, Wen Y, Zeng Z, Zhou D, Jin F
Aging 2024 Feb 19;16(4):3837-3855

Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis.

Ren Y, Zheng J, Cao Y, Zhu Y, Ling Z, Zhang Z, Huang M
Archives of oral biology 2024 Dec;168:106066

Preventive Effects of Dental Pulp Stem Cell-conditioned Media on Anti-RANKL Antibody-Related Osteonecrosis of the Jaw.

Kaminogo K, Yamaguchi S, Chen H, Yagita H, Toyama N, Urata Y, Hibi H
Calcified tissue international 2024 Aug;115(2):185-195

DKK1-SE recruits AP1 to activate the target gene DKK1 thereby promoting pancreatic cancer progression.

Shao L, Yu H, Wang M, Chen L, Ji B, Wu T, Teng X, Su M, Han X, Shi W, Hu X, Wang Z, He H, Han G, Zhang Y, Wu Q
Cell death & disease 2024 Aug 6;15(8):566

Cancer-associated fibroblast-derived Dickkopf-1 suppresses NK cell cytotoxicity in breast cancer.

Faccio R, Lee S, Ricci B, Tran J, Ye J, Clever D, Eul E, Wang J, Wong P, Ma C, Fehniger T
Research square 2024 Apr 8;

The mechanism of metformin combined with total flavonoids of Rhizoma Drynariae on ovariectomy-induced osteoporotic rats.

Jiang N, Jin H, Yang K, Zhang Z, Xu W, Chen X, Zhang Z, Xu H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Sep;165:115181

Voluntary wheel-running improved pulmonary fibrosis by reducing epithelial mesenchymal transformation.

Gao Y, Yang Z, He K, Wang Z, Zhang T, Yi J, Zhao L
Life sciences 2023 Oct 15;331:122066

Prolonged exposure to the herbicide atrazine promotes kidney fibrosis by activating Wnt/β-catenin signaling in rats.

Wang T, Huang X, Liu J, Liu W, Yang Z, He K, Chen J, Zhao L
Environmental toxicology 2023 May;38(5):1143-1152

Neuroprotective effects of oxymatrine on hypoxic-ischemic brain damage in neonatal rats by activating the Wnt/β-catenin pathway.

Lan XB, Ni YS, Liu N, Wei W, Liu Y, Yang JM, Ma L, Bai R, Zhang J, Yu JQ
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Mar;159:114266

RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression.

Dong Z, Li J, Dai W, Yu D, Zhao Y, Liu S, Li X, Zhang Z, Zhang R, Liang X, Kong Q, Jin S, Jiang H, Jiang W, Ding C
Cell death & disease 2023 Feb 7;14(2):89

Retracted Article: Exosomes from bone marrow mesenchymal stem cells promoted osteogenic differentiation by delivering miR-196a that targeted Dickkopf-1 to activate Wnt/β-catenin pathway.

Peng Z, Lu S, Lou Z, Li Z, Li S, Yang K, Li C
Bioengineered 2023 Dec;14(1):1996015

Dickkopf-1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1.

Li X, Zheng T, Zhang Y, Zhao Y, Liu F, Dai S, Liu X, Zhang M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Aug;37(8):e23048

Sirtuin 6 Overexpression Improves Rotator Cuff Tendon-to-Bone Healing in the Aged.

Moon YJ, Cui B, Cho SY, Hwang JW, Chung HC, Kwon J, Kim D, Jang KY, Kim JR, Wang SI
Cells 2023 Aug 10;12(16)

Expression of Dickkopf-1 and Twist2 in Cervical Squamous Cell Carcinoma and Their Correlation with Vasculogenic Mimicry.

Wang B, Zhang H, Wei L, Li Y
Journal of healthcare engineering 2022;2022:9288476

A novel epithelial-mesenchymal transition (EMT)-related gene signature of predictive value for the survival outcomes in lung adenocarcinoma.

Cui Y, Wang X, Zhang L, Liu W, Ning J, Gu R, Cui Y, Cai L, Xing Y
Frontiers in oncology 2022;12:974614

Cancer stem cell regulated phenotypic plasticity protects metastasized cancer cells from ferroptosis.

Wu M, Zhang X, Zhang W, Chiou YS, Qian W, Liu X, Zhang M, Yan H, Li S, Li T, Han X, Qian P, Liu S, Pan Y, Lobie PE, Zhu T
Nature communications 2022 Mar 16;13(1):1371

Nomogram Incorporating the WNT/β-Catenin Signaling Pathway for Predicting the Survival of Cutaneous Melanoma.

Zhou YX, Wang X, Pang DQ, Wang YM, Bai J, Tian F, Han D, Shi S, Hu L
International journal of general medicine 2021;14:2751-2761

ZBTB38 suppresses prostate cancer cell proliferation and migration via directly promoting DKK1 expression.

Ding G, Lu W, Zhang Q, Li K, Zhou H, Wang F, Zhao C, Fan C, Wang J
Cell death & disease 2021 Oct 25;12(11):998

miR-29a-3p-dependent COL3A1 and COL5A1 expression reduction assists sulforaphane to inhibit gastric cancer progression.

Han S, Wang Z, Liu J, Wang HD, Yuan Q
Biochemical pharmacology 2021 Jun;188:114539

Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β-catenin signaling pathway in ovariectomized rats.

Liu H, Guo Y, Zhu R, Wang L, Chen B, Tian Y, Li R, Ma R, Jia Q, Zhang H, Xia B, Li Y, Wang X, Zhu X, Zhang R, Brӧmme D, Gao S, Zhang D, Pei X
Phytotherapy research : PTR 2021 Jan;35(1):424-441

Transcriptome sequencing profiling identifies miRNA-331-3p as an osteoblast-specific miRNA in infected bone nonunion.

Zhang Y, Wang X, Huang X, Shen L, Zhang L, Shou D, Fan X
Bone 2021 Feb;143:115619

Hyperin Controls the Development and Therapy of Gastric Cancer via Regulating Wnt/β-Catenin Signaling.

Ping MH
Cancer management and research 2020;12:11773-11782

BMP8A promotes survival and drug resistance via Nrf2/TRIM24 signaling pathway in clear cell renal cell carcinoma.

Yu YP, Cai LC, Wang XY, Cheng SY, Zhang DM, Jian WG, Wang TD, Yang JK, Yang KB, Zhang C
Cancer science 2020 May;111(5):1555-1566

A1CF-promoted colony formation and proliferation of RCC depends on DKK1-MEK/ERK signal axis.

Ni D, Yi Q, Liu J, Hu Y, Lv T, Tan G, Liu Y, Xu L, Xia H, Zhou Q, Xie Y
Gene 2020 Mar 10;730:144299

LncRNA-LET inhibits cell growth of clear cell renal cell carcinoma by regulating miR-373-3p.

Ye Z, Duan J, Wang L, Ji Y, Qiao B
Cancer cell international 2019;19:311

UPLC-Q-TOF/MS characterization of efficacy substances on osteoblasts differentiation and function in rat serum after administration of Wang-Bi tablet.

Wang H, Guan Y, Wu R, Lv X, Shen X, Ye G
Biomedical chromatography : BMC 2019 Oct;33(10):e4628

Ginkgolide B and bilobalide promote the growth and increase β-catenin expression in hair follicle dermal papilla cells of American minks.

Zhang H, Shi Q, Nan W, Wang Y, Wang S, Yang F, Li G
BioFactors (Oxford, England) 2019 Nov;45(6):950-958

Distinctive role of ACVR1 in dentin formation: requirement for dentin thickness in molars and prevention of osteodentin formation in incisors of mice.

Zhang X, Shi C, Zhao H, Zhou Y, Hu Y, Yan G, Liu C, Li D, Hao X, Mishina Y, Liu Q, Sun H
Journal of molecular histology 2019 Feb;50(1):43-61

Rehmanniae Radix Preparata suppresses bone loss and increases bone strength through interfering with canonical Wnt/β-catenin signaling pathway in OVX rats.

Liu C, Wang L, Zhu R, Liu H, Ma R, Chen B, Li L, Guo Y, Jia Q, Shi S, Zhao D, Mo F, Zhao B, Niu J, Fu M, Orekhov AN, Brömme D, Gao S, Zhang D
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 2019 Feb;30(2):491-505

Depleting PTOV1 sensitizes non-small cell lung cancer cells to chemotherapy through attenuating cancer stem cell traits.

Wu Z, Liu Z, Jiang X, Mi Z, Meng M, Wang H, Zhao J, Zheng B, Yuan Z
Journal of experimental & clinical cancer research : CR 2019 Aug 6;38(1):341

A1CF-Axin2 signal axis regulates apoptosis and migration in Wilms tumor-derived cells through Wnt/β-catenin pathway.

Ni D, Liu J, Hu Y, Liu Y, Gu Y, Zhou Q, Xie Y
In vitro cellular & developmental biology. Animal 2019 Apr;55(4):252-259

BPI-9016M, a c-Met inhibitor, suppresses tumor cell growth, migration and invasion of lung adenocarcinoma via miR203-DKK1.

Zhang P, Li S, Lv C, Si J, Xiong Y, Ding L, Ma Y, Yang Y
Theranostics 2018;8(21):5890-5902

Wenyang Huazhuo Tongluo formula inhibits fibrosis via suppressing Wnt/β-catenin signaling pathway in a Bleomycin-induced systemic sclerosis mouse model.

Wang Q, Zang W, Han L, Yang L, Ye S, Ouyang J, Zhang C, Bi Y, Zhang C, Bian H
Chinese medicine 2018;13:17

Radix Salviae miltiorrhizae improves bone microstructure and strength through Wnt/β-catenin and osteoprotegerin/receptor activator for nuclear factor-κB ligand/cathepsin K signaling in ovariectomized rats.

Liu H, Zhu R, Wang L, Liu C, Ma R, Qi B, Chen B, Li L, Guo Y, Shi S, Jia Q, Niu J, Zhao D, Mo F, Gao S, Zhang D
Phytotherapy research : PTR 2018 Dec;32(12):2487-2500

Apobec-1 complementation factor regulates cell migration and apoptosis through Dickkopf1 by acting on its 3' untranslated region in MCF7 cells.

Yan X, Li Q, Ni D, Xie Y, He Q, Wan Q, Liu Y, Lyu Z, Mao Z, Zhou Q
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Jun;39(6):1010428317706218

MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC).

Liu BL, Sun KX, Zong ZH, Chen S, Zhao Y
Oncotarget 2016 Feb 9;7(6):6649-64

Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.

Ying Q, Ansong E, Diamond AM, Lu Z, Yang W, Bie X
PloS one 2015;10(5):e0126285

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human placenta tissue were subjected to SDS PAGE followed by western blot with 21112-1-AP(DKK1 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using DKK1 antibody 21112-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line