Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1765 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Dkk-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Dkk-1 in ELISAs and Western blots. In sandwich ELISAs, less than 6% cross-reactivity with recombinant human Dkk-1 and less than 0.1% cross-reactivity with recombinant mouse (rm) Dkk-2, rmDkk-3, and rmDkk-4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Multicolor quantitative confocal imaging cytometry.
Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms.
Induction of the Wnt antagonist Dickkopf-1 is involved in stress-induced hippocampal damage.
Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity.
Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone disease in multiple myeloma.
Dickkopf-1 is a master regulator of joint remodeling.
Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46
Nature methods 2018 Jan;15(1):39-46
Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms.
Himburg HA, Doan PL, Quarmyne M, Yan X, Sasine J, Zhao L, Hancock GV, Kan J, Pohl KA, Tran E, Chao NJ, Harris JR, Chute JP
Nature medicine 2017 Jan;23(1):91-99
Nature medicine 2017 Jan;23(1):91-99
Induction of the Wnt antagonist Dickkopf-1 is involved in stress-induced hippocampal damage.
Matrisciano F, Busceti CL, Bucci D, Orlando R, Caruso A, Molinaro G, Cappuccio I, Riozzi B, Gradini R, Motolese M, Caraci F, Copani A, Scaccianoce S, Melchiorri D, Bruno V, Battaglia G, Nicoletti F
PloS one 2011 Jan 27;6(1):e16447
PloS one 2011 Jan 27;6(1):e16447
Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity.
Trowbridge JJ, Guezguez B, Moon RT, Bhatia M
Stem cells (Dayton, Ohio) 2010 Aug;28(8):1379-89
Stem cells (Dayton, Ohio) 2010 Aug;28(8):1379-89
Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone disease in multiple myeloma.
Heath DJ, Chantry AD, Buckle CH, Coulton L, Shaughnessy JD Jr, Evans HR, Snowden JA, Stover DR, Vanderkerken K, Croucher PI
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2009 Mar;24(3):425-36
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2009 Mar;24(3):425-36
Dickkopf-1 is a master regulator of joint remodeling.
Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
Nature medicine 2007 Feb;13(2):156-63
Nature medicine 2007 Feb;13(2):156-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dkk-1 in Mouse Embryo. Dkk-1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Mouse Dkk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.