AF1765

antibody from R&D Systems

Targeting: DKK1 DKK-1, SK

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1765

Provider

R&D Systems

Product name

Mouse Dkk-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse Dkk-1 in ELISAs and Western blots. In sandwich ELISAs, less than 6% cross-reactivity with recombinant human Dkk-1 and less than 0.1% cross-reactivity with recombinant mouse (rm) Dkk-2, rmDkk-3, and rmDkk-4 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

O54908


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Multicolor quantitative confocal imaging cytometry.

Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46

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Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms.

Himburg HA, Doan PL, Quarmyne M, Yan X, Sasine J, Zhao L, Hancock GV, Kan J, Pohl KA, Tran E, Chao NJ, Harris JR, Chute JP
Nature medicine 2017 Jan;23(1):91-99

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Induction of the Wnt antagonist Dickkopf-1 is involved in stress-induced hippocampal damage.

Matrisciano F, Busceti CL, Bucci D, Orlando R, Caruso A, Molinaro G, Cappuccio I, Riozzi B, Gradini R, Motolese M, Caraci F, Copani A, Scaccianoce S, Melchiorri D, Bruno V, Battaglia G, Nicoletti F
PloS one 2011 Jan 27;6(1):e16447

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Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity.

Trowbridge JJ, Guezguez B, Moon RT, Bhatia M
Stem cells (Dayton, Ohio) 2010 Aug;28(8):1379-89

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Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone disease in multiple myeloma.

Heath DJ, Chantry AD, Buckle CH, Coulton L, Shaughnessy JD Jr, Evans HR, Snowden JA, Stover DR, Vanderkerken K, Croucher PI
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2009 Mar;24(3):425-36

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Dickkopf-1 is a master regulator of joint remodeling.

Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
Nature medicine 2007 Feb;13(2):156-63

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Dkk-1 in Mouse Embryo. Dkk-1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Mouse Dkk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.