Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 102-PA33 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- Anti-human DKK1
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- antibody Protein-A purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AA
PGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDE
YCASPT RGGDAGVQIC LACRKRRKRC MRHAMC
CPGN YCKNGICVSS DQNHFRGEIE ETITESFG
ND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR
SSDCASGLCC ARHFWSKICK PVLKEGQVCT K
HRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQA
SNSSRLH TCQRLEHHHH HH- Vial size
- 200 µg
- Storage
- The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Analysis with recombinant human DKK-1. Samples were loaded in 12,5% SDS-polyacrylamide gel under reducing conditions.
- Sample type
- Purified Protein
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human foreskin (cryo-section of PFA-fixed tissue) with anti-human DKK-1 (green; 5µg/ml). Nuclei counter-stained with Dapi (blue). Specimen provided by Prof. Dr. J. Wilting and Dr. K. Buttler, Goettingen. The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
- Sample type
- cryo-sections of human foreskin
