Explore
Validate
LearnPA5-18233
antibody from Invitrogen Antibodies
Targeting: PARD6A
PAR-6, PAR-6A, PAR6alpha, TAX40, TIP-40
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-18233 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAR6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.
Peng XM, Gao S, Deng HT, Cai HX, Zhou Z, Xiang R, Zhang QZ, Li LY
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Oct;32(10):5577-5586
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Oct;32(10):5577-5586
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PAR6 in human heart. Samples were incubated with PAR6 polyclonal antibody (Product # PA5-18233) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (PFA fixed) analysis of PAR6 using PAR6 Polyclonal Antibody (Product # PA5-18233) (10 µg/mL) in staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PAR6 in Jurkat cells using a PAR6 monoclonal antibody (Product # PA5-18233) at 10 µg/mL for 1hr, depicted by the blue line. The cells were paraformaldehyde fixed and permeabilized with 0.5% Triton. Primary incubation followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PAR6 in Jurkat cells using a polyclonal antibody (Product #PA5-18233). Jurkat cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.
