PA5-51995

antibody from Invitrogen Antibodies

Targeting: WIPF1 WASPIP, WIP

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51995

Provider

Invitrogen Antibodies

Product name

WIPF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: PPPPVSRNGS TSRALPATPQ LPSRSGVDSP RSGPRPPLPP DRPSAGAPPP PPPSTSIRNG FQDSPCEDEW ESRFYFHPIS DLPPPEPYVQ TTKSYPSKLA RNESRSGSNR RERGAP Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 90%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.05 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Wang C, Sample KM, Gajendran B, Kapranov P, Liu W, Hu A, Zacksenhaus E, Li Y, Hao X, Ben-David Y
Frontiers in immunology 2021;12:607836

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of WIPF1 in human skeletal muscle using WIPF1 Polyclonal Antibody (Product # PA5-51995) shows no cytoplasmic positivity in myocytes as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of WIPF1 in human tonsil using WIPF1 Polyclonal Antibody (Product # PA5-51995) shows strong cytoplasmic positivity in germinal center cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of WIPF1 in human testis using WIPF1 Polyclonal Antibody (Product # PA5-51995) shows no cytoplasmic positivity in cells in seminiferous ducts as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of WIPF1 in human spleen and pancreas tissues using WIPF1 Polyclonal Antibody (Product # PA5-51995). Corresponding WIPF1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of WIPF1 in human pancreas using WIPF1 Polyclonal Antibody (Product # PA5-51995) shows low expression as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 ShRNA mediated knockdown of WIP suppresses the percentage of CD41a and CD61 expressing HEL cells. (A) Depletion of WIP in HEL cells using three shRNAs (shRNA1-shRNA3). (B) Knockdown of WIP in HEL cells using shRNA2 (WIP-sh2), as determined by Q-RT-PCR. (C) Knockdown of WIP in HEL cells (WIPF-sh2) accelerated proliferation when compared to scrambled-transfected HEL cells. (D) Western blot of WIP-sh2 and control cells for expression of WIP, FLI1 and WASP. (E) Flow cytometry analysis of WIP-sh2 and control scrambled cells for expression of indicated megakaryocytic and erythroid markers. The unstained identical plots (left most panels) were used as negative control. (F) Summary and statistics of flow cytometry experiments (n = 3). ****P