Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX100085 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100085, RRID:AB_1951677
- Product name
- RecQ1 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE GTX100085 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A:NIH-3T37.5% SDS PAGE GTX100085 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RecQ1 antibody [C1C3] detects RecQ1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RecQ1 protein stained by RecQ1 antibody [C1C3] (GTX100085) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 8 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of RecQ1 protein from HeLa whole cell extracts using 5 £gg of RecQ1 antibody [C1C3] (GTX100085).Western blot analysis was performed using RecQ1 antibody [C1C3] (GTX100085).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RecQ1(GTX100085) antibody at 1:100 dilution.