PA5-27099

antibody from Invitrogen Antibodies

Targeting: RECQL RecQ1, RecQL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27099

Provider

Invitrogen Antibodies

Product name

RecQ1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, HeLa, A375. Predicted reactivity: Mouse (80%), Pig (81%), Bovine (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RecQ1 Polyclonal Antibody detects RecQ1 protein by Western blot analysis. A. 30 µg A431 whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract. C. 30 µg A375 whole cell lysate/extract.7.5 % SDS-PAGE. RecQ1 Polyclonal Antibody (Product # PA5-27099) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RecQ1 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a RecQ1 polyclonal antibody (Product # PA5-27099) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RecQ1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RecQ1 Polyclonal Antibody (Product # PA5-27099) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RecQ1 Polyclonal Antibody detects RecQ1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RecQ1 Polyclonal Antibody (Product # PA5-27099) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RecQ1 was performed in HeLa whole cell extracts using 5 µg of RecQ1 Polyclonal Antibody (Product # PA5-27099). Samples were transferred to a membrane and probed with RecQ1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.