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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27100 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RECQ1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3. Predicted reactivity: Pig (82%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RECQ1 Polyclonal Antibody (Product # PA5-27100). Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. RECQ1 Polyclonal Antibody (Product # PA5-27100) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RECQ1 Polyclonal Antibody (Product # PA5-27100). Sample (30 µg of whole cell lysate). Lane A: 293T . 7.5% SDS PAGE. RECQ1 Polyclonal Antibody (Product # PA5-27100) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RECQ1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RECQ1 Polyclonal Antibody (Product # PA5-27100) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 8 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RecQ1 (Product # PA5-27100) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RecQ1 was performed in HeLa whole cell extracts using 5 µg of RECQ1 Polyclonal Antibody (Product # PA5-27100). Samples were transferred to a membrane and probed with RECQ1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
