BS8389

antibody from Bioworld Technology, Inc
Targeting: RPN1 OST1
Provider product page for BS8389
Western blot
Immunohistochemistry

Antibody data

Product number
BS8389 - Provider product page
Provider
Bioworld Technology, Inc
Product name
RPN1 polyclonal antibody
Antibody type
Polyclonal
Antigen
Recombinant full length Human RPN1.
Description
The proteasome is a multisubunit protease responsible for degrading proteins conjugated to ubiquitin. Substrates are targeted for proteolysis by the ubiquitin pathway by the addition of a polyubiquitin chain before being degraded by the 26S proteasome. The 26S proteasome is made up of two subunits, the 20S proteolytic subunit and the 19S regulatory particle, which recognizes and unfolds ubiquitinated proteins. The ubiquitinlike domains (Ubls) of Ubiquitin-like proteins Rad23 and Dsk2 bind both polyubiquitin chains and the 19S regulatory particle (RP) of the 26S proteasome . The Ubl domain mediates the binding of Rad23 to proteasomes, which in turn promotes DNA repair and modulates protein degradation, possibly by delivering ubiquitinylated cargo to proteasomes . Rad23 binds proteasomes by directly interacting with the base subcomplex of the regulatory particle of the proteasome . A component of the base, Rpn1, specifically recognizes the UBL domain of Rad23 through its leucine-rich-repeat-like (LRR-like) domain. Rpn1, for regulatory-particle non-ATPase subunit 1, is one of the largest subunits of proteasome . Base subunit Rpn10, however, does not mediate the binding of UBL proteins to the proteasome in yeast, although it can contribute to the binding of ubiquitin chains by intact proteasomes .
Reactivity
Human, Mouse, Rat
Host
Rabbit
Isotype
IgG
Vial size
100ul
Concentration
1 mg/ml
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
RPN1 protein structure - BS8389 shown in red.