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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA503653 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503653, RRID:AB_11127777
- Product name
- RPN1 (Ribophorin I) mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)
- Antibody type
- Monoclonal
- Description
- RPN1 (Ribophorin I) mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RPN1
- Isotype
- IgG
- Antibody clone number
- OTI2G9
- Vial size
- 100 µl
- Concentration
- 0.35 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPN1 (RC201554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPN1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-RPN1 monoclonal antibody at 1:200.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201554 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPN1 antibody(TA503653), and then analyzed by flow cytometry.
- Validation comment
- FC
