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LearnMA5-24263
antibody from Invitrogen Antibodies
Targeting: PLXNA2
FLJ11751, FLJ30634, KIAA0463, OCT, PLXN2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
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- Product number
- MA5-24263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Plexin A2 Monoclonal Antibody (583603)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human Plexin A4, B1, B2, D1, recombinant mouse Plexin A1, A3, B3, or C1 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 583603
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of mouse brain tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-mouse Plexin A2 Monoclonal Antibody (Product # MA5-24263) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for Plexin A2 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Plexin A2 in mouse brain tissue. Samples were incubated in Plexin A2 monoclonal antibody (Product # MA5-24263) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for Plexin A2 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-mouse Plexin A2 Monoclonal Antibody (Product # MA5-24263) at 10 µg/mL for 3 hours at room temperature. Cells were stained using a 557-conjugated Anti-Rat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Plexin A2 in immersion fixed HUVEC human umbilical vein endothelial cells. Samples were incubated in Plexin A2 monoclonal antibody (Product # MA5-24263) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Plexin A2 in immersion fixed bEnd.3 mouse endothelioma cell line. Samples were incubated in Plexin A2 monoclonal antibody (Product # MA5-24263) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.
