PA5-47746

antibody from Invitrogen Antibodies

Targeting: PLXNA2 FLJ11751, FLJ30634, KIAA0463, OCT, PLXN2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47746

Provider

Invitrogen Antibodies

Product name

Plexin A2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A3, and recombinant human Plexin B1 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

PLXNA2 and LRRC40 as candidate genes in autism spectrum disorder.

Pijuan J, Ortigoza-Escobar JD, Ortiz J, Alcalá A, Calvo MJ, Cubells M, Hernando-Davalillo C, Palau F, Hoenicka J
Autism research : official journal of the International Society for Autism Research 2021 Jun;14(6):1088-1100

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-human/mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Plexin A2 in immersion fixed HUVEC human umbilical vein endothelial cells. Samples were incubated in Plexin A2 polyclonal antibody (Product # PA5-47746) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Plexin A2 in immersion fixed bEnd.3 mouse endothelioma cell line. Samples were incubated in Plexin A2 polyclonal antibody (Product # PA5-47746) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Plexin A2 in bEnd.3 mouse endothelioma cell line. Samples were incubated in Plexin A2 polyclonal antibody (Product # PA5-47746) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

3 FIGURE PlxnA2 and LRRC40 variants affect the function of these proteins. (a) PlxnA2 subcellular localization in control and patient fibroblasts. (b) Percentage of nuclear/cytoplasmic or cytoplasmic localization patterns (box plot represents 25 th percentile, median, and 75 th percentile. The whiskers extend to the minimum and maximum values; n = 3 independent biological replicates; at least 100 random cells per experiment). Mantel-Haenszel chi 2 test (*** p

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4 FIGURE PlxnA2 interacts with LRRC40. (a) Schematic representation of protein interaction network based on STRING software. (b) PLA of control and patient fibroblasts using alpha-PlxnA2 and alpha-LRRC40. (c) Quantification of PLA dots per cell (box plots represent 25 th percentile, median, 75 th percentile. The whiskers extend to the minimum and maximum values; n = 3 independent biological replicates; n = 60 cells). Mann-Whitney U -test (*** p