- PA5-47746 - Invitrogen Antibodies PLXNA2 antibody

Submitted references PLXNA2 and LRRC40 as candidate genes in autism spectrum disorder.

Pijuan J, Ortigoza-Escobar JD, Ortiz J, Alcalá A, Calvo MJ, Cubells M, Hernando-Davalillo C, Palau F, Hoenicka J
Autism research : official journal of the International Society for Autism Research 2021 Jun;14(6):1088-1100

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis from lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Plexin A2 at approximately 246 kDa (as indicated) using 10 µg/mL of Goat Anti-human/mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47746) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions and using the66-440 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis from lysates of bEnd.3 mouse endothelioma cell line and rat embryonic cortical neuron cells. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human/mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47746) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Plexin A2 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Plexin A2 in bEnd.3 mouse endothelioma cell line and rat embryonic cortical neuron cells. Samples were incubated in Plexin A2 polyclonal antibody (Product # PA5-47746) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Plexin A2 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Plexin A2 in 0.2 mg/mL lysates of bEnd.3 mouse endothelioma cell line. Samples were incubated in Plexin A2 polyclonal antibody (Product # PA5-47746) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 0.0763888888888889. A specific band was detected for Plexin A2 at approximately 246 kDa (as indicated). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemical analysis of Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-human/mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Plexin A2 in immersion fixed HUVEC human umbilical vein endothelial cells. Samples were incubated in Plexin A2 polyclonal antibody (Product # PA5-47746) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of Plexin A2 in immersion fixed bEnd.3 mouse endothelioma cell line. Samples were incubated in Plexin A2 polyclonal antibody (Product # PA5-47746) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Plexin A2 in bEnd.3 mouse endothelioma cell line. Samples were incubated in Plexin A2 polyclonal antibody (Product # PA5-47746) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 FIGURE PlxnA2 and LRRC40 variants affect the function of these proteins. (a) PlxnA2 subcellular localization in control and patient fibroblasts. (b) Percentage of nuclear/cytoplasmic or cytoplasmic localization patterns (box plot represents 25 th percentile, median, and 75 th percentile. The whiskers extend to the minimum and maximum values; n = 3 independent biological replicates; at least 100 random cells per experiment). Mantel-Haenszel chi 2 test (*** p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 4 FIGURE PlxnA2 interacts with LRRC40. (a) Schematic representation of protein interaction network based on STRING software. (b) PLA of control and patient fibroblasts using alpha-PlxnA2 and alpha-LRRC40. (c) Quantification of PLA dots per cell (box plots represent 25 th percentile, median, 75 th percentile. The whiskers extend to the minimum and maximum values; n = 3 independent biological replicates; n = 60 cells). Mann-Whitney U -test (*** p

PA5-47746

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