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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- HPA022275 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA022275, RRID:AB_1853944
- Product name
- Anti-MAP1B
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human MAP1B, Gene description: microtubule-associated protein 1B, Alternative Gene Names: MAP5, PPP1R102, Validated applications: ICC, IHC, WB, Uniprot ID: P46821, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Multi-omics profiling identifies a deregulated FUS-MAP1B axis in ALS/FTD–associated UBQLN2 mutants
The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability
The molecular chaperone Hsp90α deficiency causes retinal degeneration by disrupting Golgi organization and vesicle transportation in photoreceptors
Stromal contribution to the colorectal cancer transcriptome
RNA Deep Sequencing as a Tool for Selection of Cell Lines for Systematic Subcellular Localization of All Human Proteins
Strohm L, Hu Z, Suk Y, Rühmkorf A, Sternburg E, Gattringer V, Riemenschneider H, Berutti R, Graf E, Weishaupt J, Brill M, Harbauer A, Dormann D, Dengjel J, Edbauer D, Behrends C
Life Science Alliance 2022;5(11):e202101327
Life Science Alliance 2022;5(11):e202101327
The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability
Yao X, Liao K, Zheng X, Ding Y, Wu Y
Journal of Molecular Cell Biology 2020;12(6):448-461
Journal of Molecular Cell Biology 2020;12(6):448-461
The molecular chaperone Hsp90α deficiency causes retinal degeneration by disrupting Golgi organization and vesicle transportation in photoreceptors
Liao K, Liu T, Wei Z, Zhou M, Ding Y, Zheng X, Wu Y
Journal of Molecular Cell Biology 2020;12(3):216-229
Journal of Molecular Cell Biology 2020;12(3):216-229
Stromal contribution to the colorectal cancer transcriptome
Isella C, Terrasi A, Bellomo S, Petti C, Galatola G, Muratore A, Mellano A, Senetta R, Cassenti A, Sonetto C, Inghirami G, Trusolino L, Fekete Z, De Ridder M, Cassoni P, Storme G, Bertotti A, Medico E
Nature Genetics 2015;47(4):312-319
Nature Genetics 2015;47(4):312-319
RNA Deep Sequencing as a Tool for Selection of Cell Lines for Systematic Subcellular Localization of All Human Proteins
Danielsson F, Wiking M, Mahdessian D, Skogs M, Ait Blal H, Hjelmare M, Stadler C, Uhlén M, Lundberg E
Journal of Proteome Research 2012;12(1):299-307
Journal of Proteome Research 2012;12(1):299-307
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting MAP1B or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting MAP1B Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:103
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MAP1B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using HPA022275 antibody. Corresponding MAP1B RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
