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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-42827 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-42827, RRID:AB_2137405
- Product name
- Rabbit Polyclonal MAP1B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Phosphorylated Thr1265 on MAP1B (Thr1261 in rat).
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.12 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nerve growth factor promotes reorganization of the axonal microtubule array at sites of axon collateral branching.
Loss of MAP function leads to hippocampal synapse loss and deficits in the Morris Water Maze with aging.
The ubiquitin ligase Mindbomb 1 coordinates gastrointestinal secretory cell maturation.
MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates Its Association to the Microtubule Network.
Evolution of the spatial distribution of MAP1B phosphorylation sites in vertebrate neurons.
Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons.
Glycogen synthase kinase-3beta phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons.
Ketschek A, Jones S, Spillane M, Korobova F, Svitkina T, Gallo G
Developmental neurobiology 2015 Dec;75(12):1441-61
Developmental neurobiology 2015 Dec;75(12):1441-61
Loss of MAP function leads to hippocampal synapse loss and deficits in the Morris Water Maze with aging.
Ma QL, Zuo X, Yang F, Ubeda OJ, Gant DJ, Alaverdyan M, Kiosea NC, Nazari S, Chen PP, Nothias F, Chan P, Teng E, Frautschy SA, Cole GM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 May 21;34(21):7124-36
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 May 21;34(21):7124-36
The ubiquitin ligase Mindbomb 1 coordinates gastrointestinal secretory cell maturation.
Capoccia BJ, Jin RU, Kong YY, Peek RM Jr, Fassan M, Rugge M, Mills JC
The Journal of clinical investigation 2013 Apr;123(4):1475-91
The Journal of clinical investigation 2013 Apr;123(4):1475-91
MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates Its Association to the Microtubule Network.
Marchbank K, Waters S, Roberts RG, Solomon E, Whitehouse CA
International journal of cell biology 2012;2012:208014
International journal of cell biology 2012;2012:208014
Evolution of the spatial distribution of MAP1B phosphorylation sites in vertebrate neurons.
Tymanskyj SR, Lin S, Gordon-Weeks PR
Journal of anatomy 2010 Jun;216(6):692-704
Journal of anatomy 2010 Jun;216(6):692-704
Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons.
Scales TM, Lin S, Kraus M, Goold RG, Gordon-Weeks PR
Journal of cell science 2009 Jul 15;122(Pt 14):2424-35
Journal of cell science 2009 Jul 15;122(Pt 14):2424-35
Glycogen synthase kinase-3beta phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons.
Trivedi N, Marsh P, Goold RG, Wood-Kaczmar A, Gordon-Weeks PR
Journal of cell science 2005 Mar 1;118(Pt 5):993-1005
Journal of cell science 2005 Mar 1;118(Pt 5):993-1005
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Western blot analysis of p62, Nbr1, MAP1B-HC, and MAP1B-LC1 protein levels following blockage of autophagic protein degradation. Phospho-MAP1B-HC is degraded by autophagy. Upon blockage of autophagic degradation with Bafilomycin A1: (Baf), levels of phospho-MAP1B-HC increase compared with levels of total MAP1B-HC. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/ijcb/2012/208014/), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Immunohistochemical analysis of rat spinal cord transverse section from an E12 embryo.
