Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-81261 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-81261, RRID:AB_11042280
- Product name
- Rabbit Polyclonal MAP1B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MAP1B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Stromal contribution to the colorectal cancer transcriptome.
RNA deep sequencing as a tool for selection of cell lines for systematic subcellular localization of all human proteins.
Isella C, Terrasi A, Bellomo SE, Petti C, Galatola G, Muratore A, Mellano A, Senetta R, Cassenti A, Sonetto C, Inghirami G, Trusolino L, Fekete Z, De Ridder M, Cassoni P, Storme G, Bertotti A, Medico E
Nature genetics 2015 Apr;47(4):312-9
Nature genetics 2015 Apr;47(4):312-9
RNA deep sequencing as a tool for selection of cell lines for systematic subcellular localization of all human proteins.
Danielsson F, Wiking M, Mahdessian D, Skogs M, Ait Blal H, Hjelmare M, Stadler C, Uhlén M, Lundberg E
Journal of proteome research 2013 Jan 4;12(1):299-307
Journal of proteome research 2013 Jan 4;12(1):299-307
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP1B Antibody [NBP1-81261] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MAP1B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human cerebral cortex shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining in human cerebral cortex and pancreas tissues using anti-MAP1B antibody. Corresponding MAP1B RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human cerebellum shows moderate to strong membranous positivity in cells in granular layer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human skeletal muscle shows low positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.