Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30584 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAP1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, P4.5 mouse brain(24ug), Neuro-2a(15ug). Predicted reactivity: Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAP1B using 30 µg of U87-MG lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a MAP1B polyclonal antibody (Product # PA5-30584) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAP1B was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAP1B Polyclonal Antibody (Product # PA5-30584) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MAP1B Polyclonal Antibody (Product # PA5-30584). Sample (15 µg of lysate). Lane A: P4.5 mouse brain. 5% SDS PAGE. MAP1B Polyclonal Antibody (Product # PA5-30584) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
