Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 617002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#617002, RRID:AB_315862
- Product name
- Purified anti-a-Adducin
- Antibody type
- Polyclonal
- Antigen
- Recombinant (partial)
- Description
- The α-adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca2+/calmodulin and acts as a substrate for protein kinases A and C. α-addducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers α/β, and α/γ. The molecular weights for the various isoforms are α=120 kD; β=110kD; γ=80kD. This protein has been reported to promote the assembly of the spectrin-actin network. Polymorphisms in α-adducin are associated with cardiovascular disease and hypertension. α-adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kinase, α-adducin can regulate membrane ruffling and cell motility. The Poly6170 antibody has been shown to be useful for Western blotting of mouse and human α-adducin protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references Rac GTPases regulate the morphology and deformability of the erythrocyte cytoskeleton.
Kalfa TA, Pushkaran S, Mohandas N, Hartwig JH, Fowler VM, Johnson JF, Joiner CH, Williams DA, Zheng Y
Blood 2006 Dec 1;108(12):3637-45
Blood 2006 Dec 1;108(12):3637-45
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-α-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated