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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27460 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Adducin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurakt, Raji. Predicted reactivity: Mouse (95%), Rat (96%), Zebrafish (89%), Xenopus laevis (90%), Chicken (92%), Rhesus Monkey (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.02 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha Adducin Polyclonal Antibody (Product # PA5-27460). Sample (30 µg of whole cell lysate). Lane A: Jurakt . 7.5% SDS PAGE. alpha Adducin Polyclonal Antibody (Product # PA5-27460) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha Adducin in methanol-fixed A431 cells using an Alpha Adducin polyclonal antibody (Product # PA5-27460) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Adducin Polyclonal Antibody detects alpha Adducin protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alpha Adducin Polyclonal Antibody (Product # PA5-27460) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Adducin Polyclonal Antibody detects alpha Adducin protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Alpha Adducin Polyclonal Antibody (Product # PA5-27460) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
