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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29641 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Adducin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, NCI-H929, mouse liver. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (89%), Xenopus laevis (93%), Chicken (98%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha Adducin Polyclonal Antibody (Product # PA5-29641). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. alpha Adducin Polyclonal Antibody (Product # PA5-29641) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Adducin Polyclonal Antibody detects alpha Adducin protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.7.5 % SDS-PAGE. Alpha Adducin Polyclonal Antibody (Product # PA5-29641) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SG xenograft , using Alpha-adducin (Product # PA5-29641) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
