sc-25825

antibody from Santa Cruz Biotechnology

Targeting: HPSE HPA, HPSE1, HSE1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-25825

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-25825, RRID:AB_2120854

Product name

Anti-HPSE

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

Heparanase activity in alveolar and embryonal rhabdomyosarcoma: implications for tumor invasion.

Masola V, Maran C, Tassone E, Zin A, Rosolen A, Onisto M
BMC cancer 2009 Aug 28;9:304

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Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells.

Hong X, Jiang F, Kalkanis SN, Zhang ZG, Zhang X, Zheng X, Jiang H, Mikkelsen T, Chopp M
Journal of experimental & clinical cancer research : CR 2008 Jul 22;27(1):23

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 213, 118, 83, 50, 33, 26, 18

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 61

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.