Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- sc-25825 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-25825, RRID:AB_2120854
- Product name
- Anti-HPSE
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Heparanase activity in alveolar and embryonal rhabdomyosarcoma: implications for tumor invasion.
Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells.
Masola V, Maran C, Tassone E, Zin A, Rosolen A, Onisto M
BMC cancer 2009 Aug 28;9:304
BMC cancer 2009 Aug 28;9:304
Increased chemotactic migration and growth in heparanase-overexpressing human U251n glioma cells.
Hong X, Jiang F, Kalkanis SN, Zhang ZG, Zhang X, Zheng X, Jiang H, Mikkelsen T, Chopp M
Journal of experimental & clinical cancer research : CR 2008 Jul 22;27(1):23
Journal of experimental & clinical cancer research : CR 2008 Jul 22;27(1):23
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 213, 118, 83, 50, 33, 26, 18
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 61
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.