PA5-95775

antibody from Invitrogen Antibodies

Targeting: HPSE HPA, HPSE1, HSE1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-95775

Provider

Invitrogen Antibodies

Product name

HPa1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: Jurkat, BxPC-3, A-431, mouse spleen, mouse lung, rat spleen, rat lung; Cellular Location: Lysosome membrane, Nucleus, Peripheral membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.17 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cells, using HPSE Polyclonal antibody (Product # PA5-95775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HPa1 in extracts of various cells using HPa1 Polyclonal Antibody (Product # PA5-95775) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HPa1 was performed in HeLa cells using HPa1 Polyclonal Antibody (Product # PA5-95775).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HPa1 was performed in HeLa cells using HPa1 Polyclonal Antibody (Product # PA5-95775).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HPa1 was performed in NIH/3T3 cells using HPa1 Polyclonal Antibody (Product # PA5-95775).