PA5-95775

antibody from Invitrogen Antibodies

Targeting: HPSE HPA, HPSE1, HSE1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-95775

Provider

Invitrogen Antibodies

Product name

HPa1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: Jurkat, BxPC-3, A-431, mouse spleen, mouse lung, rat spleen, rat lung; Cellular Location: Lysosome membrane, Nucleus, Peripheral membrane protein Immunogen sequence: QDVVDLDFFT QEPLHLVSPS FLSVTIDANL ATDPRFLILL GSPKLRTLAR GLSPAYLRFG GTKTDFLIFD PKKESTFEER SYWQSQVNQD ICKYGSIPPD VEEKLRLEWP YQEQLLLREH YQKKFKNSTY SRSSVDVLYT FANCSGLDLI FGLNALLRTA DLQWNSSNAQ LLLDYCSSKG YNISWELGNE PNSFLKKADI FINGSQLGED FIQLHKLLRK STFKNAKLYG PDVGQPRRKT

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.89 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cells, using HPSE Polyclonal antibody (Product # PA5-95775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HPa1 in extracts of various cells using HPa1 Polyclonal Antibody (Product # PA5-95775) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HPa1 was performed in HeLa cells using HPa1 Polyclonal Antibody (Product # PA5-95775).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HPa1 was performed in HeLa cells using HPa1 Polyclonal Antibody (Product # PA5-95775).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HPa1 was performed in NIH/3T3 cells using HPa1 Polyclonal Antibody (Product # PA5-95775).