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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V7352 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Spectrin beta III Antibody / SPTBN2
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Spectrin beta III antibody is suitable for use in Flow cytometry/ELISA/Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- SPTBN2/2894R
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant Spectrin beta III antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human PANC-1 and HeLa cell lysate with recombinant Spectrin beta III antibody (clone SPTBN2/2894R). Predicted molecular weight ~246 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human pancreatic cancer with recombinant Spectrin beta III antibody (clone SPTBN2/2894R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human pancreatic cancer with recombinant Spectrin beta III antibody (clone SPTBN2/2894R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HeLa cells with recombinant Spectrin beta III antibody (blue) and isotype control (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant Spectrin beta III antibody (clone SPTBN2/2894R) as confirmation of integrity and purity.
