Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- V7355 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Spectrin beta III Antibody / SPTBN2
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Spectrin beta III antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- SPTBN2/2887R
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant Spectrin beta III antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human PANC-1 and HeLa cell lysate with recombinant Spectrin beta III antibody (clone SPTBN2/2887R). Predicted molecular weight ~246 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human HeLa cells with recombinant Spectrin beta III antibody (green, clone SPTBN2/2887R) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HeLa cells with recombinant Spectrin beta III antibody (blue) and isotype control (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant Spectrin beta III antibody (clone SPTBN2/2887R) as confirmation of integrity and purity.
