21632-1-AP

antibody from Proteintech Group

Targeting: KIF5B KNS, KNS1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21632-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21632-1-AP, RRID:AB_11182931

Product name

KIF5B antibody

Antibody type

Polyclonal

Description

KD/KO validated KIF5B antibody (Cat. #21632-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo.

Smith ER, Turner ED, Abdelhamid MAS, Craggs TD, Twelvetrees AE
iScience 2026 Mar 20;29(3):114875

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Progranulin-driven lysosomal acidification facilitates exocytosis of PHEV-hijacked lysosomes for viral release.

Wang Z, Chen Y, He W, Liu Y, Jiao Y, Wang G, Guan J, Zhao K, Zhang Q, Gao F, Li Z, Lan Y
mBio 2026 Jan 14;17(1):e0290325

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Excessive vigorous exercise impairs cognitive function through a muscle-derived mitochondrial pretender.

Huang Y, Hu B, Liu Y, Xie LQ, Dai Y, An YZ, Peng XY, Cheng YL, Guo YF, Kuang WH, Xiao Y, Chen X, Zheng YJ, Xie GQ, Wang JP, Peng H, Luo XH
Cell metabolism 2026 Feb 3;38(2):281-297.e11

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RPL41 inhibits the proliferation and migration of retinoblastoma through the ARL5B-associated lysosomal trafficking.

Li Y, Zhan T, Chen T, Zhong Q, Xiao S, Wang A, Jiao Y
Frontiers in immunology 2025;16:1704080

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Kinesin-1 coordinates cross-talk between microtubule and actin cytoskeletons during dendritic cell migration.

Duquesne P, Aoun C, Kurowska M, Perot BP, Zhang K, Debili M, Weimershaus M, Mauvais FX, Cagnard N, Goudin N, Medel B, Montero-Hermández JE, Diedhiou L, Huang JD, Fischer A, de Saint Basile G, Ménager MM, Vargas P, Sepulveda FE, Ménasché G
Science advances 2025 Oct 17;11(42):eadx7672

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Metformin-Loaded Tannic Acid Nanoparticles Attenuate Osteoarthritis by Promoting Chondrocyte Mitochondria Homeostasis Based on Mitocytosis.

Da W, Deng X, Chen Q, Yang Y, Jiang S, Chen X, Lu G, Shen B
Biomacromolecules 2025 Mar 10;26(3):1507-1519

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OSBP Participates in Neural Damage Repair by Regulating Lysosome Transport Under Oxidative Stress.

Fei M, Luo S, Gao C, Huang X, Wang L, Jin T, Liu M, Zhou M, Wang H
Molecular neurobiology 2025 Jun;62(6):7557-7575

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Regulation of Axonal Microtubule Polarity Orientation in Different Kinds of Neurons.

Patil A, Guha S, Isiltan I, Muralidharan H, Madugula K, Baas PW
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jun 15;39(11):e70683

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Oxyresveratrol suppressed melanogenesis, dendrite formation, and melanosome transport in melanocytes via regulation of the MC1R/cAMP/MITF pathway.

Zhang J, Liu S, Guo W, Huang Y, Li N
Scientific reports 2025 Jul 1;15(1):20400

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KIF5A regulates axonal repair and time-dependent axonal transport of SFPQ granules and mitochondria in human motor neurons.

Guerra San Juan I, Brunner JW, Eggan K, Toonen RF, Verhage M
Neurobiology of disease 2025 Jan;204:106759

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RAF1 kinase contributes to autophagic lysosome reformation.

Toifl S, Didusch S, Ehrenreiter K, Desideri E, Dorard C, Baccarini M
Cell reports 2025 Apr 22;44(4):115490

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Advanced nerve regeneration enabled by neural conformal electronic stimulators enhancing mitochondrial transport.

Bai H, Zhang S, Yang H, Wang J, Chen H, Li J, Li L, Yang Q, Peng B, Zhu Z, Ni S, Liu K, Lei W, Tao TH, Feng Y
Bioactive materials 2024 Sep;39:287-301

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KIF5A regulates axonal repair and time-dependent axonal transport of SFPQ granules and mitochondria in human motor neurons.

Guerra San Juan I, Brunner J, Eggan K, Toonen RF, Verhage M
bioRxiv : the preprint server for biology 2024 Sep 11;

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Casein kinase 1 inhibitor avoids TDP-43 pathology propagation in a patient-derived cellular model of amyotrophic lateral sclerosis.

Cuevas EP, Martinez-Gonzalez L, Gordillo C, Tosat-Bitrián C, Pérez de la Lastra C, Sáenz A, Gil C, Palomo V, Martin-Requero Á, Martinez A
Neurobiology of disease 2024 Mar;192:106430

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Static magnetic field-modulated mesenchymal stem cell-derived mitochondria-containing microvesicles for enhanced intervertebral disc degeneration therapy.

Shi P, Gao H, Cheng Z, Zhao K, Chen Y, Chen X, Gan W, Zhang A, Yang C, Zhang Y
Journal of nanobiotechnology 2024 Jul 31;22(1):457

---

Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI.

Liu G, He M, Wu C, Lv P, Sun H, Wang H, Xin X, Liao H
Experimental neurology 2024 Feb;372:114618

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Ablation of KIF2C in Purkinje cells impairs metabotropic glutamate receptor trafficking and motor coordination in male mice.

Zheng R, Xu FX, Zhou L, Xu J, Shen Y, Hao K, Wang XT, Deng J
The Journal of physiology 2023 Sep;601(17):3905-3920

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LncRNA BCAN-AS1 stabilizes c-Myc via N(6)-methyladenosine-mediated binding with SNIP1 to promote pancreatic cancer.

Wu G, Su J, Zeng L, Deng S, Huang X, Ye Y, Li R, Bai R, Zhuang L, Li M, Zhou Q, Zheng Y, Deng J, Zhang S, Chen R, Lin D, Zhang J, Zheng J
Cell death and differentiation 2023 Oct;30(10):2213-2230

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Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36.

Zhu Z, Li X, Wang X, Zuo X, Ma Y, Gao X, Liang Z, Zhang Z, Song Z, Ding T, Ju C, Li P, Li K, Zhang J, Quan H, Wang Z, Hu X
Bioengineering & translational medicine 2023 May;8(3):e10473

---

Activation of TLR7-mediated autophagy increases epileptic susceptibility via reduced KIF5A-dependent GABA(A) receptor transport in a murine model.

Liu J, Ke P, Guo H, Gu J, Liu Y, Tian X, Wang X, Xiao F
Experimental & molecular medicine 2023 Jun;55(6):1159-1173

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Kinesin-1 Regulates Endocytic Trafficking of Classical Swine Fever Virus along Acetylated Microtubules.

Lou JX, Liu YY, Bai JS, Cheng Y, Zhang J, Liu CC, Zhou B
Journal of virology 2023 Jan 31;97(1):e0192922

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Mast cell granule motility and exocytosis is driven by dynamic microtubule formation and kinesin-1 motor function.

Ibanga J, Zhang EL, Eitzen G, Guo Y
PloS one 2022;17(3):e0265122

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Decreased mitochondrial function in UVA-irradiated dermal fibroblasts causes the insufficient formation of type I collagen and fibrillin-1 fibers.

Katsuyama Y, Yamawaki Y, Sato Y, Muraoka S, Yoshida M, Okano Y, Masaki H
Journal of dermatological science 2022 Oct;108(1):22-29

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GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Bellio C, Emperador M, Castellano P, Gris-Oliver A, Canals F, Sánchez-Pla A, Zamora E, Arribas J, Saura C, Serra V, Tabernero J, Littlefield BA, Villanueva J
Cancers 2022 May 23;14(10)

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Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense.

Wells KM, He K, Pandey A, Cabello A, Zhang D, Yang J, Gomez G, Liu Y, Chang H, Li X, Zhang H, Feng X, da Costa LF, Metz R, Johnson CD, Martin CL, Skrobarczyk J, Berghman LR, Patrick KL, Leibowitz J, Ficht A, Sze SH, Song J, Qian X, Qin QM, Ficht TA, de Figueiredo P
eLife 2022 May 19;11

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Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Kang Y, Yin S, Liu J, Jiang Y, Huang Z, Chen L, Shao L
Journal of hazardous materials 2022 Feb 15;424(Pt D):127660

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Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport.

Chen XQ, Das U, Park G, Mobley WC
Alzheimer's research & therapy 2021 Mar 10;13(1):59

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MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.

Wang F, Chen X, Cheng H, Song L, Liu J, Caplan S, Zhu L, Wu JY
EMBO reports 2021 Jul 5;22(7):e52006

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Dynamic Dissection of Dynein and Kinesin-1 Cooperatively Mediated Intercellular Transport of Porcine Epidemic Diarrhea Coronavirus along Microtubule Using Single Virus Tracking.

Hou W, Kang W, Li Y, Shan Y, Wang S, Liu F
Virulence 2021 Dec;12(1):615-629

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Enhanced autophagic retrograde axonal transport by dynein intermediate chain upregulation improves Aβ clearance and cognitive function in APP/PS1 double transgenic mice.

Zhou F, Xiong X, Li S, Liang J, Zhang X, Tian M, Li X, Gao M, Tang L, Li Y
Aging 2020 Jun 24;12(12):12142-12159

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Immunolocalization of dynein, dynactin, and kinesin in the cerebral tissue as a possible supplemental diagnostic tool for traumatic brain injury in postmortem examination.

Olczak M, Poniatowski Ł, Kwiatkowska M, Samojłowicz D, Tarka S, Wierzba-Bobrowicz T
Folia neuropathologica 2019;57(1):51-62

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Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.

Wang Q, Tian J, Chen H, Du H, Guo L
Neurobiology of disease 2019 Jul;127:410-418

---

Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.

Franchini DM, Lanvin O, Tosolini M, Patras de Campaigno E, Cammas A, Péricart S, Scarlata CM, Lebras M, Rossi C, Ligat L, Pont F, Arimondo PB, Laurent C, Ayyoub M, Despas F, Lapeyre-Mestre M, Millevoi S, Fournié JJ
Cell reports 2019 Jan 2;26(1):94-107.e7

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Kinesin-1 Is a New Actor Involved in Platelet Secretion and Thrombus Stability.

Adam F, Kauskot A, Kurowska M, Goudin N, Munoz I, Bordet JC, Huang JD, Bryckaert M, Fischer A, Borgel D, de Saint Basile G, Christophe OD, Ménasché G
Arteriosclerosis, thrombosis, and vascular biology 2018 May;38(5):1037-1051

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Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features.

Pesaresi M, Giatti S, Spezzano R, Romano S, Diviccaro S, Borsello T, Mitro N, Caruso D, Garcia-Segura LM, Melcangi RC
Biology of sex differences 2018 Jan 19;9(1):6

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Kinesin-1 controls mast cell degranulation and anaphylaxis through PI3K-dependent recruitment to the granular Slp3/Rab27b complex.

Munoz I, Danelli L, Claver J, Goudin N, Kurowska M, Madera-Salcedo IK, Huang JD, Fischer A, González-Espinosa C, de Saint Basile G, Blank U, Ménasché G
The Journal of cell biology 2016 Oct 24;215(2):203-216

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Transport of c-MYC by Kinesin-1 for proteasomal degradation in the cytoplasm.

Lee CM
Biochimica et biophysica acta 2014 Sep;1843(9):2027-36

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The KIF5B antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human KIF5B. This antibody recognizes human,mouse,rat antigen. The KIF5B antibody has been validated for the following applications: ELISA, IHC, WB analysis.

Appendix

21632-1-AP.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 21632-1-AP(KIF5B antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 21632-1-AP(KIF5B antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue