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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- MA5-38502 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calponin 3 Monoclonal Antibody (5E4E4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody has been tested in indirect ELISA.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5E4E4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Calponin 3 using Calponin 3 Monoclonal Antibody (Product # MA5-38502) in HeLa (1), U251 (2), and HEK293 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using Calponin 3 Monoclonal Antibody (Product # MA5-38502) against human CNN3 (AA: 26-130) recombinant protein. (Expected MW is 37.6 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Calponin 3 using Calponin 3 Monoclonal Antibody (Product # MA5-38502) in HEK293 (1) and CNN3 (AA: 26-130)-human IgG-Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Calponin 3 was performed by loading 20 µg of HAP1 WT (lane 1) and HAP1 Calponin 3 KO (lane 2) cell lysates in RIPA buffer (Product # 89901) onto a 4-20% gradient polyacrylamide gel (Product # WXP42012BOX). Proteins on the blot were visualized with Ponceau staining (below immunoblot) (Product # BP10310). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. Calponin 3 was detected at approximately 36 kDa using Calponin 3 Monoclonal Antibody (5E4E4) (Product # MA5-38502) at a dilution of 1:1,000 in 5% BSA in TBST overnight at 4 degree celsius. The blot was probed with Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Product # 62-6520) diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr at room temperature. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106) and iBright™ CL1500 Imaging System (Product # A44240). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of Calponin 3 in HeLa cells using Calponin 3 Monoclonal Antibody (Product # MA5-38502) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of Calponin 3 in HeLa cells using Calponin 3 Monoclonal Antibody (Product # MA5-38502) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Calponin 3 in paraffin-embedded cervical cancer tissues using Calponin 3 Monoclonal Antibody (Product # MA5-38502) and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Calponin 3 in paraffin-embedded rectum cancer tissues using Calponin 3 Monoclonal Antibody (Product # MA5-38502) and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Calponin 3 in paraffin-embedded cervical cancer tissues using Calponin 3 Monoclonal Antibody (Product # MA5-38502) and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Calponin 3 in paraffin-embedded rectum cancer tissues using Calponin 3 Monoclonal Antibody (Product # MA5-38502) and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Calponin 3 in HeLa cells using Calponin 3 Monoclonal Antibody (Product # MA5-38502) (green) and negative control (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Calponin 3 in HeLa cells using Calponin 3 Monoclonal Antibody (Product # MA5-38502) (green) and negative control (red).
