Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28375 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calponin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2, NIH-3T3, JC.
- Concentration
- 0.86 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Calponin 3 Polyclonal Antibody (Product # PA5-28375). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: HeLa. Lane C: HepG2. 10% SDS PAGE. Calponin 3 Polyclonal Antibody (Product # PA5-28375) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Calponin 3 Polyclonal Antibody (Product # PA5-28375). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3 . Lane B: JC . 10% SDS PAGE. Calponin 3 Polyclonal Antibody (Product # PA5-28375) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Calponin 3 Polyclonal Antibody (Product # PA5-28375). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calponin 3 Polyclonal Antibody (Product # PA5-28375) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Calponin 3 was performed in paraffin-embedded rat ovary tissue using Calponin 3 Polyclonal Antibody (Product # PA5-28375) at a dilution of 1:500.
