PA5-103890

antibody from Invitrogen Antibodies

Targeting: HOXB3 HOX2, HOX2G

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-103890

Provider

Invitrogen Antibodies

Product name

HOXB3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total HOXB3.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/β-Catenin Pathway.

Yu Z, Liu J, Fan Q, Yu J, Ren X, Wang X
Analytical cellular pathology (Amsterdam) 2022;2022:9302496

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HOXB3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HOXB3 polyclonal antibody (Product # PA5-103890) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HOXB3 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with HOXB3 polyclonal antibody (Product # PA5-103890) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HOXB3 in rat brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with HOXB3 polyclonal antibody (Product # PA5-103890) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 HOXB3 is found to be a downstream target of miR-375. (a) Binding sites between miR-375 and HOXB3 predicted through an online website. (b) The target relationship between miR-375 and HOXB3 was confirmed by dual-luciferase reporter gene assay. (c) HOXB3 mRNA expression in SNU-449 and Huh7 cells using RT-qPCR. (d, e) HOXB3 protein expression in SNU-449 and Huh7 cells using Western blot assay. (f) Enrichment analysis of miR-375 targeted genes using KEGG enrichment analysis. * p < 0.05 vs. the NC mimic group; # p < 0.05 vs. the EV-NC mimic group. All data were expressed as mean +- standard deviation of at least three independent experiments. Data analysis among multiple groups was performed by two-way ANOVA, followed by Tukey's multiple comparison test.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 HOXB3 is found to be a downstream target of miR-375. (a) Binding sites between miR-375 and HOXB3 predicted through an online website. (b) The target relationship between miR-375 and HOXB3 was confirmed by dual-luciferase reporter gene assay. (c) HOXB3 mRNA expression in SNU-449 and Huh7 cells using RT-qPCR. (d, e) HOXB3 protein expression in SNU-449 and Huh7 cells using Western blot assay. (f) Enrichment analysis of miR-375 targeted genes using KEGG enrichment analysis. * p < 0.05 vs. the NC mimic group; # p < 0.05 vs. the EV-NC mimic group. All data were expressed as mean +- standard deviation of at least three independent experiments. Data analysis among multiple groups was performed by two-way ANOVA, followed by Tukey's multiple comparison test.