PAB28538

antibody from Abnova Corporation

Targeting: IDH3G

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28538

Provider

Abnova Corporation

Product name

IDH3G polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant IDH3G.

Antigen sequence

ADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHK
SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDIL
IVRENTEGEYSSLEHESVAGVVESLKIITKAKSLR
IAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQ
CCREVAA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: EFO-21, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with IDH3G polyclonal antibody (Cat # PAB28538) at 1:250-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with IDH3G polyclonal antibody (Cat # PAB28538) at 1:100-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with IDH3G polyclonal antibody (Cat # PAB28538) at 1-4 ug/ml shows positivity in nucleoli and mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human IDH3G polyclonal antibody (Cat # PAB28538) shows strong cytoplasmic positivity in cortical cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)