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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-51385 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IDH3G Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FDVMVMPNLY GNIVNNVCAG LVGGPGLVAG ANYGHVYAVF ETATRNTGKS IANKNIANPT ATLLASCMML DHLKLHSYAT SIRKAVLASM DNENMHTPDI GGQGTTSEAI QDVI Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IDH3G in Lane 1: Marker (kDa) 219, 112, 85, 49, 32, 25, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human cell line A-431. Samples were probed using an IDH3G Polyclonal Antibody (Product # PA5-51385).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IDH3G in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells); Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla). Samples were probed using an IDH3G Polyclonal Antibody (Product # PA5-51385).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IDH3G in human cell line U-251 MG shows positivity in mitochondria & nucleus but excluded from the nucleoli. Samples were probed using an IDH3G Polyclonal Antibody (Product # PA5-51385).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IDH3G in human cerebral cortex and pancreas tissues using IDH3G Polyclonal Antibody (Product # PA5-51385). Corresponding IDH3G RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IDH3G in human fallopian tube using IDH3G Polyclonal Antibody (Product # PA5-51385) shows weak to moderate granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IDH3G in human duodenum using IDH3G Polyclonal Antibody (Product # PA5-51385) shows moderate granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IDH3G in human pancreas using IDH3G Polyclonal Antibody (Product # PA5-51385) shows weak positivity in exocrine glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IDH3G in human cerebral cortex using IDH3G Polyclonal Antibody (Product # PA5-51385) shows moderate granular cytoplasmic positivity in neuronal cells.
