RQ8518

antibody from NSJ Bioreagents

Targeting: IDH3G

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ8518

Provider

NSJ Bioreagents

Product name

IDH3G Antibody / Isocitrate dehydrogenase [NAD] subunit gamma

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Vial size

100 ug

Storage

After reconstitution, the IDH3G antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat heart, 2) rat kidney, 3) rat brain, 4) mouse heart, 5) mouse kidney, 6) mouse brain and 7) rat HBZY cell lysate with IDH3G antibody. Predicted molecular weight ~41 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human A431, 2) human MCF7, 3) human HeLa, 4) human 293T, 5) human U-251 and 6) monkey COS-7 cell lysate with IDH3G antibody. Predicted molecular weight ~41 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human PC-3 cells with IDH3G antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human esophageal squamous carcinoma tissue with IDH3G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human rectum adenocarcinoma tissue with IDH3G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with IDH3G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placental tissue with IDH3G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat heart tissue with IDH3G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human 293T cells with IDH3G antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IDH3G antibody.