Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324372 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal RORA Antibody (T216)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal RORA Antibody (T216)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RORA
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.36 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-RORA Antibody (T216) (Cat.#TA324372) in A2058 cell line lysates (35ug/lane). RORA (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with RORA Antibody (T216)(Cat#TA324372).Hela cells were incubated with RORA primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . RORA immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
