PA5-11224

antibody from Invitrogen Antibodies

Targeting: RORA NR1F1, ROR1, ROR2, ROR3, RZRA

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-11224

Provider

Invitrogen Antibodies

Product name

RORA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

RORα is critical for mTORC1 activity in T cell-mediated colitis.

Chi X, Jin W, Bai X, Zhao X, Shao J, Li J, Sun Q, Su B, Wang X, Yang XO, Dong C
Cell reports 2021 Sep 14;36(11):109682

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RORα Regulates Cholesterol Metabolism of CD8(+) T Cells for Anticancer Immunity.

Lee IK, Song H, Kim H, Kim IS, Tran NL, Kim SH, Oh SJ, Lee JM
Cancers 2020 Jun 29;12(7)

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Exosomal miRNA-19a and miRNA-614 Induced by Air Pollutants Promote Proinflammatory M1 Macrophage Polarization via Regulation of RORα Expression in Human Respiratory Mucosal Microenvironment.

Shin CH, Byun J, Lee K, Kim B, Noh YK, Tran NL, Park K, Kim SH, Kim TH, Oh SJ
Journal of immunology (Baltimore, Md. : 1950) 2020 Dec 1;205(11):3179-3190

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RORα is crucial for attenuated inflammatory response to maintain intestinal homeostasis.

Oh SK, Kim D, Kim K, Boo K, Yu YS, Kim IS, Jeon Y, Im SK, Lee SH, Lee JM, Ko Y, Lee H, Park D, Fang S, Baek SH
Proceedings of the National Academy of Sciences of the United States of America 2019 Oct 15;116(42):21140-21149

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a RORA polyclonal antibody (Product # PA5-11224). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a RORA polyclonal antibody (Product # PA5-11224) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 RORalpha potentiates T cell cholesterol synthesis via NF-kappaB inhibition. ( A ) NF-kappaB-luciferase reporter-expressed Jurkat cells were treated with SR1078 or SR3335 at doses of 1, 5, 10, and 20 muM for 1 day. Luciferase was reacted by the Luciferase Assay System and measured using a microplate reader. The p -value was calculated by a t -test (* p < 0.05, ** p < 0.01, *** p < 0.001). ( B ) Co-immunoprecipitation assays between RORalpha and p50, NF-kappaB component (left). The binding affinity of RORalpha WT or C90A with p50 was assessed in 293T cells (middle). The binding between RORalpha and p50 was assessed with SR3335 treatment in 293T cells (right) ( C ) The introduction of RORalphaC90A decreased the transcriptional activation of the 5XRORE luciferase reporter (left). Compared to RORE, the introduction of RORalpha WT, C90A, and DeltaAF2 mutants had the same effect of transrepression on the 3XNFkappaB luciferase reporter (middle and right). The p -value was calculated by a t -test (** p < 0.01, *** p < 0.001). ( D ) Effects of RORalpha knockdown on 3XNFkappaB luciferase reporter in 293T cells treated with LPS (shRORalpha, +: 0.5mug/muL, ++: 2mug/muL). ( E ) The illustration represents the location of NF-kappaB elements on Acat1 and Abca1 promoter. Proposed model of RORalpha recruitment with HDAC3 and binding on the NF-kappaB target promoters (left). ChIP assays on Acat1 or Abca1 promoters in WT, ROR KO ( sg ), or p50 KO MEFs (right). The p -value was calculate