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- Validations
- Western blot [2]
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- Product number
- PA5-11224 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RORA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.42 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references RORα is critical for mTORC1 activity in T cell-mediated colitis.
RORα Regulates Cholesterol Metabolism of CD8(+) T Cells for Anticancer Immunity.
Exosomal miRNA-19a and miRNA-614 Induced by Air Pollutants Promote Proinflammatory M1 Macrophage Polarization via Regulation of RORα Expression in Human Respiratory Mucosal Microenvironment.
RORα is crucial for attenuated inflammatory response to maintain intestinal homeostasis.
Chi X, Jin W, Bai X, Zhao X, Shao J, Li J, Sun Q, Su B, Wang X, Yang XO, Dong C
Cell reports 2021 Sep 14;36(11):109682
Cell reports 2021 Sep 14;36(11):109682
RORα Regulates Cholesterol Metabolism of CD8(+) T Cells for Anticancer Immunity.
Lee IK, Song H, Kim H, Kim IS, Tran NL, Kim SH, Oh SJ, Lee JM
Cancers 2020 Jun 29;12(7)
Cancers 2020 Jun 29;12(7)
Exosomal miRNA-19a and miRNA-614 Induced by Air Pollutants Promote Proinflammatory M1 Macrophage Polarization via Regulation of RORα Expression in Human Respiratory Mucosal Microenvironment.
Shin CH, Byun J, Lee K, Kim B, Noh YK, Tran NL, Park K, Kim SH, Kim TH, Oh SJ
Journal of immunology (Baltimore, Md. : 1950) 2020 Dec 1;205(11):3179-3190
Journal of immunology (Baltimore, Md. : 1950) 2020 Dec 1;205(11):3179-3190
RORα is crucial for attenuated inflammatory response to maintain intestinal homeostasis.
Oh SK, Kim D, Kim K, Boo K, Yu YS, Kim IS, Jeon Y, Im SK, Lee SH, Lee JM, Ko Y, Lee H, Park D, Fang S, Baek SH
Proceedings of the National Academy of Sciences of the United States of America 2019 Oct 15;116(42):21140-21149
Proceedings of the National Academy of Sciences of the United States of America 2019 Oct 15;116(42):21140-21149
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a RORA polyclonal antibody (Product # PA5-11224) in A2058 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-RORA Polyclonal Antibody (Product # PA5-11224) and a 70 kDa band corresponding to RORA was observed in HepG2 upon Cobalt chloride treatment. Nuclear enriched extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 (Cobalt chloride (100 µM, 24hours)) (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a RORA polyclonal antibody (Product # PA5-11224). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a RORA polyclonal antibody (Product # PA5-11224) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 RORalpha potentiates T cell cholesterol synthesis via NF-kappaB inhibition. ( A ) NF-kappaB-luciferase reporter-expressed Jurkat cells were treated with SR1078 or SR3335 at doses of 1, 5, 10, and 20 muM for 1 day. Luciferase was reacted by the Luciferase Assay System and measured using a microplate reader. The p -value was calculated by a t -test (* p < 0.05, ** p < 0.01, *** p < 0.001). ( B ) Co-immunoprecipitation assays between RORalpha and p50, NF-kappaB component (left). The binding affinity of RORalpha WT or C90A with p50 was assessed in 293T cells (middle). The binding between RORalpha and p50 was assessed with SR3335 treatment in 293T cells (right) ( C ) The introduction of RORalphaC90A decreased the transcriptional activation of the 5XRORE luciferase reporter (left). Compared to RORE, the introduction of RORalpha WT, C90A, and DeltaAF2 mutants had the same effect of transrepression on the 3XNFkappaB luciferase reporter (middle and right). The p -value was calculated by a t -test (** p < 0.01, *** p < 0.001). ( D ) Effects of RORalpha knockdown on 3XNFkappaB luciferase reporter in 293T cells treated with LPS (shRORalpha, +: 0.5mug/muL, ++: 2mug/muL). ( E ) The illustration represents the location of NF-kappaB elements on Acat1 and Abca1 promoter. Proposed model of RORalpha recruitment with HDAC3 and binding on the NF-kappaB target promoters (left). ChIP assays on Acat1 or Abca1 promoters in WT, ROR KO ( sg ), or p50 KO MEFs (right). The p -value was calculate
