Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-61100 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61100, RRID:AB_925252
- Product name
- Rabbit Polyclonal Aquaporin-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for ~29k AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~ 37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references AQP2 Abundance is Regulated by the E3-Ligase CHIP Via HSP70.
Negative feedback from CaSR signaling to aquaporin-2 sensitizes vasopressin to extracellular Ca2.
Vasopressin increases S261 phosphorylation in AQP2-P262L, a mutant in recessive nephrogenic diabetes insipidus.
Centrone M, Ranieri M, Di Mise A, Berlingerio SP, Russo A, Deen PMT, Staub O, Valenti G, Tamma G
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(2):515-531
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(2):515-531
Negative feedback from CaSR signaling to aquaporin-2 sensitizes vasopressin to extracellular Ca2.
Ranieri M, Tamma G, Di Mise A, Russo A, Centrone M, Svelto M, Calamita G, Valenti G
Journal of cell science 2015 Jul 1;128(13):2350-60
Journal of cell science 2015 Jul 1;128(13):2350-60
Vasopressin increases S261 phosphorylation in AQP2-P262L, a mutant in recessive nephrogenic diabetes insipidus.
Trimpert C, van den Berg DT, Fenton RA, Klussmann E, Deen PM
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2012 Dec;27(12):4389-97
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2012 Dec;27(12):4389-97
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).
