Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PB9474 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Aquaporin 2/AQP2 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for AQUAPORIN 2/AQP2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. AQUAPORIN 2/AQP2 information: Molecular Weight: 28837 MW; Subcellular Localization: Apical cell membrane ; Multi-pass membrane protein . Basolateral cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane ; Multi- pass membrane protein . Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein . Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated; Tissue Specificity: Expressed in renal collecting tubules.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Quantitative proteomics reveals TMOD1-related proteins associated with water balance regulation.
Lagopsis supina exerts its diuretic effect via inhibition of aquaporin-1, 2 and 3 expression in a rat model of traumatic blood stasis.
Expression and significance of aquaporin-2 and serum hormones in placenta of patients with preeclampsia.
Protective effects of propofol on endotoxemia-induced acute kidney injury in rats.
Effect of exercise training on renal function and renal aquaporin-2 expression in rats with chronic heart failure.
The changes of aquaporin 2 in the graft of acute rejection rat renal transplantation model.
Wang W, Xu D, Zhong L, Zhang W, Kang J, Zhou J, Ka W, Sun D, Xia X, Xie L, Yao W
PloS one 2019;14(7):e0219932
PloS one 2019;14(7):e0219932
Lagopsis supina exerts its diuretic effect via inhibition of aquaporin-1, 2 and 3 expression in a rat model of traumatic blood stasis.
He J, Zeng L, Wei R, Zhong G, Zhu Y, Xu T, Yang L
Journal of ethnopharmacology 2019 Mar 1;231:446-452
Journal of ethnopharmacology 2019 Mar 1;231:446-452
Expression and significance of aquaporin-2 and serum hormones in placenta of patients with preeclampsia.
Zhao Y, Lin L, Lai A
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2018 Jan;38(1):42-48
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2018 Jan;38(1):42-48
Protective effects of propofol on endotoxemia-induced acute kidney injury in rats.
Cui WY, Tian AY, Bai T
Clinical and experimental pharmacology & physiology 2011 Nov;38(11):747-54
Clinical and experimental pharmacology & physiology 2011 Nov;38(11):747-54
Effect of exercise training on renal function and renal aquaporin-2 expression in rats with chronic heart failure.
Lin QQ, Lin R, Ji QL, Zhang JY, Wang WR, Yang LN, Zhang KF
Clinical and experimental pharmacology & physiology 2011 Mar;38(3):179-85
Clinical and experimental pharmacology & physiology 2011 Mar;38(3):179-85
The changes of aquaporin 2 in the graft of acute rejection rat renal transplantation model.
Chen B, Zang CS, Zhang JZ, Wang WG, Wang JG, Zhou HL, Fu YW
Transplantation proceedings 2010 Jun;42(5):1884-7
Transplantation proceedings 2010 Jun;42(5):1884-7
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Aquaporin 2 using anti-Aquaporin 2 antibody (PB9474). Aquaporin 2 was detected in paraffin-embedded section of Human Renal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aquaporin 2 Antibody (PB9474) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image