A302-754A-T

antibody from Invitrogen Antibodies

Targeting: GTF2E1 FE, TFIIE-A

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-754A-T

Provider

Invitrogen Antibodies

Product name

GTF2E1/TFIIE-alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

1 mg/mL

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GTF2E1/TFIIE-alpha by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- GTF2E1/TFIIE-alpha (Cat. No. A302-754A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human GTF2E1/TFIIE-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody A302-754A used for IP at 3 µg/mg lysate. GTF2E1/TFIIE-alpha was also immunoprecipitated by rabbit anti-GTF2E1/TFIIE-alpha antibody A302-755A, which recognizes a downstream epitope. For blotting immunoprecipitated GTF2E1/TFIIE-alpha, A302-755A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.