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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-87533 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SFPQ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MMIRQREMEE QMRRQREESY SRMGYMDPRE RDMRMGGGGA MNMGDPYGSG GQKFPPLGGG GGIGYEANPG VPPATMSGSM MGSDMRTERF GQGGAGPVGG QGPRGMGPGT PAGYGRGREE YEGPNKKPRF; Positive Samples: OVCAR3, HeLa, Jurkat, HepG2, Mouse brain, Mouse liver, Mouse spleen, Rat brain; Cellular Location: Cytoplasm, Nucleus matrix
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SFPQ in extracts of various cell lines using SFPQ Polyclonal Antibody (Product # PA5-87533) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using SFPQ Polyclonal antibody (Product # PA5-87533) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SFPQ was performed in HeLa cells using SFPQ Polyclonal Antibody (Product # PA5-87533) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SFPQ in paraffin-embedded rat brain using SFPQ Polyclonal Antibody (Product # PA5-87533) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SFPQ in paraffin-embedded human lung cancer using SFPQ Polyclonal Antibody (Product # PA5-87533) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SFPQ in paraffin-embedded mouse lung using SFPQ Polyclonal Antibody (Product # PA5-87533) at a dilution of 1:100.
