RQ7421

antibody from NSJ Bioreagents

Targeting: SFPQ PPP1R140, PSF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7421

Provider

NSJ Bioreagents

Product name

PSF Antibody / PTB-associated-splicing factor / SFPQ

Antibody type

Polyclonal

Description

This highly specific PSF antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the PSF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human 293T, 3) human HepG2, 4) human PC-3, 5) rat brain, 6) mouse brain, 7) mouse C2C12 and 8) mouse NIH 3T3 cell lysate with PSF antibody. Predicted molecular weight ~76 kDa, commonly observed at 90-100 kDa with possible 47 kDa and 68 kDa cleavage products.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human MCF7 cells with PSF antibody (green) and Alpha Tubulin antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human ovarian cancer tissue with PSF antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE mouse brain cancer tissue with PSF antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human ovarian cancer tissue with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human squamous cell carcinoma of the penis with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse brain with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat brain with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human SiHa cells with PSF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSF antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse ANA-1 cells with PSF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSF antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of rat RH35 cells with PSF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSF antibody.