PA5-29948

antibody from Invitrogen Antibodies

Targeting: SFPQ PPP1R140, PSF

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29948

Provider

Invitrogen Antibodies

Product name

SFPQ Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (85%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.78 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SFPQ was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SFPQ Polyclonal Antibody (Product # PA5-29948) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SFPQ was performed in 293T whole cell extracts using 5 µg of SFPQ Polyclonal Antibody (Product # PA5-29948). Samples were transferred to a membrane and probed with SFPQ Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.