sc-30062

PLCD1 antibody from Santa Cruz Biotechnology

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for sc-30062

Antibody Data

Product number

sc-30062

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-30062, RRID:AB_2163388

Product name

Anti-PLCD1

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Suppression of PLCbeta2 by endotoxin plays a role in the adenosine A(2A) receptor-mediated switch of macrophages from an inflammatory to an angiogenic phenotype.
Grinberg S, Hasko G, Wu D, Leibovich SJ
The American journal of pathology 2009 Dec;175(6):2439-53

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 213, 118, 83, 50, 33, 26, 18

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 86

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.