AM1832b

antibody from Abgent

Targeting: MMP14 MT1-MMP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1832b

Provider

Abgent

Proper citation

Abgent Cat#AM1832b, RRID:AB_10663143

Product name

MMP14 Antibody (C-term) (Ascites)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgM

Vial size

100 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of MMP14 Antibody (C-term) (Cat. #AM1832b) in A2058 cell line lysates (35ug/lane).MMP14 (arrow) was detected using the purified Mab.(1:1000)

Primary Ab dilution

1:1000~16000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

MMP14 Antibody (C-term)(Ascites) (Cat. #AM1832b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MMP14 Antibody (C-term)(Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

MMP14 Antibody (C-term)(Ascites) (Cat. #AM1832b) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50