Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF918 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal MMP-14/MT1-MMP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human MMP-14/MT1-MMP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, -12, -13, -15, and -16 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Site-specific expression of gelatinolytic activity during morphogenesis of the secondary palate in the mouse embryo.
Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent signaling.
Gkantidis N, Blumer S, Katsaros C, Graf D, Chiquet M
PloS one 2012;7(10):e47762
PloS one 2012;7(10):e47762
Tumor cell invasion of collagen matrices requires coordinate lipid agonist-induced G-protein and membrane-type matrix metalloproteinase-1-dependent signaling.
Fisher KE, Pop A, Koh W, Anthis NJ, Saunders WB, Davis GE
Molecular cancer 2006 Dec 8;5:69
Molecular cancer 2006 Dec 8;5:69
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of MMP-14/MT1-MMP in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Goat Anti-Human MMP-14/MT1-MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF918, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).