Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M00656 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for MT1 MMP/MMP14 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat MT1 MMP/MMP14 information: Molecular Weight: 65894 MW; Subcellular Localization: Membrane ; Single-pass type I membrane protein . Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm; Tissue Specificity: Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- BFD-13
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Norcantharidin inhibits tumor growth and vasculogenic mimicry of human gallbladder carcinomas by suppression of the PI3-K/MMPs/Ln-5γ2 signaling pathway.
MicroRNA-10b promotes migration and invasion through KLF4 and HOXD10 in human bladder cancer.
Zhang JT, Sun W, Zhang WZ, Ge CY, Liu ZY, Zhao ZM, Lu XS, Fan YZ
BMC cancer 2014 Mar 15;14:193
BMC cancer 2014 Mar 15;14:193
MicroRNA-10b promotes migration and invasion through KLF4 and HOXD10 in human bladder cancer.
Xiao H, Li H, Yu G, Xiao W, Hu J, Tang K, Zeng J, He W, Zeng G, Ye Z, Xu H
Oncology reports 2014 Apr;31(4):1832-8
Oncology reports 2014 Apr;31(4):1832-8
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kdieny, using MMP14 Antibody (M00656)MMP14 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MMP14 Antibody (M00656)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image