F54844

antibody from NSJ Bioreagents

Targeting: MMP14 MT1-MMP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54844

Provider

NSJ Bioreagents

Product name

MMP14 Antibody

Antibody type

Polyclonal

Description

This highly specific MMP14 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the MMP14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human A2058, 2) human HT-1080, 3) human MCF-7, 4) human NCI-H292, 5) mouse kidney and 6) rat kidney tissue lysate with MMP14 antibody. Predicted molecular weight ~66 kDa (pro form), ~54 kDa (active form), ~44 kDa (inactive form).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lung adenocarcinoma tissue with MMP14 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MCF7 cells with MMP14 antibody; Blue=isotype control, Green= MMP14 antibody.