PA5-13183

antibody from Invitrogen Antibodies

Targeting: MMP14 MT1-MMP

Provider product page for PA5-13183

Western blot

Antibody data

Antibody Data
Antigen structure
References [4]
Comments [0]
Validations
Western blot [3]
Immunocytochemistry [1]
Immunohistochemistry [1]
Flow cytometry [1]
Other assay [1]

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Product number: PA5-13183 - Provider product page
Provider: Invitrogen Antibodies
Product name: MMP14 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with bovine, mouse, porcine and rat based on sequence homology.
Reactivity: Human, Mouse
Host: Rabbit
Vial size: 400 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

MMP14 protein structure - PA5-13183 shown in red.

Submitted references Mesenchymal stromal cell-derived septoclasts resorb cartilage during developmental ossification and fracture healing.

Sivaraj KK, Majev PG, Jeong HW, Dharmalingam B, Zeuschner D, Schröder S, Bixel MG, Timmen M, Stange R, Adams RH
Nature communications 2022 Jan 28;13(1):571

The Effect of Neddylation Inhibition on Inflammation-Induced MMP9 Gene Expression in Esophageal Squamous Cell Carcinoma.

Hryniewicz-Jankowska A, Wierzbicki J, Tabola R, Stach K, Sossey-Alaoui K, Augoff K
International journal of molecular sciences 2021 Feb 9;22(4)

TNF-α promotes breast cancer cell migration and enhances the concentration of membrane-associated proteases in lipid rafts.

Wolczyk D, Zaremba-Czogalla M, Hryniewicz-Jankowska A, Tabola R, Grabowski K, Sikorski AF, Augoff K
Cellular oncology (Dordrecht) 2016 Aug;39(4):353-63

Serous and mucinous ovarian tumors express different profiles of MMP-2, -7, -9, MT1-MMP, and TIMP-1 and -2.

Brun JL, Cortez A, Commo F, Uzan S, Rouzier R, Daraï E
International journal of oncology 2008 Dec;33(6):1239-46

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MMP14 using a MMP14 polyclonal antibody (Product # PA5-13183) in A375 lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MMP14 (arrow) using a MMP14 polyclonal antibody (Product # PA5-13183) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HT-1080 (Lane 2), A549 (Lane 3), A-375 (Lane 4), MCF7 (Lane 5), Mouse Lung (Lane 6), Mouse Colon (Lane 7) and Mouse Liver (Lane 8). The blot was probed with Anti-MMP14 Polyclonal Antibody (Product # PA5-13183, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 65kDa band corresponding to MMP14 was observed across all the cell lines and tissue lysates tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of MMP14 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MMP14 Polyclonal Antibody (Product # PA5-13183) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing golgi and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a MMP14 polyclonal antibody (Product # PA5-13183), followed by HRP-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of MCF-7 cells using a MMP14 polyclonal antibody (Product # PA5-13183) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Effect of tumor necrosis factor-alpha (TNF-alpha) and MLN4924 on matrix metalloproteinase 9 (MMP9) expression and esophageal squamous cell carcinoma (ESCC) cell migration. ( A ) After overnight serum starvation, KYSE150 cells were pretreated or not with 1 muM MLN4924 for 30 min and then stimulated with TNF-alpha at the indicated concentration for 24 h. Activity of MMP9 and MMP2 was analyzed by gelatin zymography in the conditioned media. The protein levels of membrane type I-matrix metalloproteinase (MT1-MMP), cyclin dependent kinase inhibitor 1A (CDKN1A/p21), c-Jun, nuclear factor kappa B (NFkappaB) and SP1 were determined by Western blotting. Fold change calculated as the ratio of relative levels of proteins normalized to beta-actin (ACTB) between the cells treated with TNF-alpha in combination with MLN4924 and the cells treated with TNF-alpha alone is shown in Figure S4 . Values shown are means +- SEM: whiskers: min-max. ( B ) MMP9 messenger RNA (mRNA) expression was analyzed by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) in both KYSE150 and KYSE70 cells treated for 24 h with TNF-alpha (30 ng/mL) and MLN4924 (1 µM) alone or in combination. Results are presented as fold change of MMP9 gene in the treated cells relative to the untreated controls normalized to the expression of the reference gene encoding glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ). ( C ) Representative images from wound healing assay showing the chan