MA1033

antibody from Invitrogen Antibodies

Targeting: CXCL11 b-R1, H174, I-TAC, IP-9, SCYB11, SCYB9B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1033

Provider

Invitrogen Antibodies

Product name

Anti-CXCL11 Monoclonal Antibody (22F1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. The Molecular weight is ~8303 Da from positions 22 - 94 as determined by MALDI.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

22F1

Vial size

200 µg

Concentration

1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human ITAC was performed by loading 0.25 µg of recombinant human ITAC and induced HeLa cell culture supernatant onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with an anti -human ITAC monoclonal antibody (Product # MA1032, clone 10C6, MA1033: clone 22F1) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product # 34075).

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA analysis of Human ITAC was performed using the Thermo Scientific ELISA Kit (Product # EHITAC) by coating a blank 96-well microtiter plate with 100 µL per well of Human ITAC moncolonal antibody (Product # MA1032) in duplicate at 1, 3, 4, 5, 7, and 9 µg/mL in DPBS (Product # 28374) and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300 µL per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human ITAC recombinant protein at 50 µL per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/mL for 2 hours at room temperature along with 50 µL of Human ITAC biotinylated monoclonal antibody (Product # MA1033B) in all applicable wells at 0.2 µg/mL for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer (Product # N503) and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100 µL per well of TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid (Product # N600). Absorbances were read on a spectrophotometer at 450-550 nm.